Aspergillus fumigatus is an opportunistic and allergenic pathogenic fungus, responsible for about 65% of fungal infections in humans and for several pathologies in immunocompromised individuals, including invasive aspergillosis (IA). A. fumigatus has four MAPKs described: MpkA (central regulator of the CWI signaling pathway and acting in response to oxidative stress); MpkB (not well characterized); MpkC and SakA (orthologs of S. cerevisiae- Hog1, a kinase involved with the control of osmolarity glycerol response (HOG) pathway). Current studies are interested in the understand of the role of MAPKs in the cellular signaling triggered by treatment with stress-inductor molecules, as sorbitol or congo-red and caspofungin. It was recently demonstrated the involvement of Hog1 orthologous: SakA and MpkC, in the response to different cellular stresses as osmotic and oxidative stress, and in the fungus adaptation to caspofungin. In addition, MpkA has been related with the pathogen response to the caspofungin stress. The new gene edition strategies for the obtention of phosphomimetic mutants, as CRISPR-cas 9 for example, are important tools for the study of signaling pathways elements modulated by phosphorylation during a stress suffered by organisms. In this way, these BEPE project has as major aim to understand the cellular signaling mediated by phosphorylation of proteins involving the pathways of SakA and MpkC of A. fumigatus.
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