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Effect of the mgtC gene-deletion on Salmonella gallinarum pathogenicity in susceptible poultry

Grant number: 18/12614-7
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): October 01, 2018
Effective date (End): December 31, 2021
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Pathology
Principal researcher:Angelo Berchieri Junior
Grantee:Lucas Bocchini Rodrigues Alves
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Associated scholarship(s):19/25091-5 - Requirement of mgtC gene for Salmonella gallinarum survival ability within macrophages, BE.EP.DR

Abstract

Salmonella enterica subsp. enterica serovar Gallinarum biovar Gallinarum (S. Gallinarum) is the etiological agent of fowl typhoid, an infectious-disease that affects birds of any age and which for some years has been identified as one of the major health challenges to poultry farming. Such concern is due to being a systemic disease of acute clinical course with high mortality rate and of difficult control. However, majority of the studies conducted with Salmonella spp. to better understand the parasite-host interaction in typhoidal pictures were done with specific-host serovars of humans and mice, S. Typhi and S. Typhimurium, respectively. In those cases, during systemic disease the bacteria expresses the mgtC gene, encoded within the chromosomal region known as "Salmonella Pathogenicity Island - 3" (SPI-3). The mgtC is apparently involved in typhoidal serovars survival and replication within macrophages. Thus, mgtC-depleted are attenuate, being one of the main SPI-3 genes. Until now, there is no literature on the need of genes codified within SPI-3 to S. Gallinarum pathogenicity. During our previous study was observed that lacking phoPQ genes attenuated S. Gallinarum, leading to believe that one of the factors that cause this circumstance would be the expression-failure of genes enconded within SPI-3, regulated by PhoPQ system. In the light of this hypothesis, in the present study a mutant strain of S. Gallinarum with mgtC-depleted gene (SG mgtC) will be constructed. Moreover, the ability of the SG mgtC strain to multiply within a growth medium that similar to the macrophage interior and its pathogenicity to susceptible poutry will be evaluated. (AU)

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