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Synthesis and morphological characterization of chitosan hydrogel/glycophosphate hydrated disodium salt with polycaprolactone nanofibers

Grant number: 18/14662-9
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): October 01, 2018
Effective date (End): September 30, 2019
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Alexandre Luiz Souto Borges
Grantee:Geovanna Coutinho Andria
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

Currently, the synthesis of the hydrogels based on the chitosan biopolymer (Ch), aim to obtain biomaterials with properties suitable for tissue regeneration and controlled release of drugs. In this sense, the objective of this study is to synthesize and morphologically characterize the hydrogel of chitosan and glycerophosphate (GP) hydrated disodium salt associated with electrospun polycaprolactone nanofibers (PCL) for application in tissue regeneration. For this, initially, a solution will be made by dissolving 3g of PCL in 2mL of Acetone. Afterward, the solution will be subjected to the electrospinning process, obtaining polymeric nanofibers, arranged at the bottom of a petri dish. Subsequently, two solutions are prepared: one of Ch, where 0.4 g of Ch in 19 mL of acetic acid will be dissolved, and one of GP, dissolving 1.7 g of GP in 1.7 mL of PBS. The GP solution will be mixed slowly to the Ch, under constant magnetic stirring. The final solution (ChGp) will be placed in the petri dish with PCL fibers and placed under refrigeration for 24h. The material is then subjected to the process of total evaporation of the solvent under ambient conditions for another 24 hours. The obtained material will be characterized according to its morphological properties. Thus, it is expected to find a pattern of synthesis and morphological characterization of a hydrogel of chitosan and hydrated disodium salt glycerophosphate associated with polycaprolactone nanofibers, for possible application in tissue regeneration and drug delivery.