Development of nanocomposite polymer filaments with anti-inflammatory properties for 3D printing as bone substituent

• Grant number: 18/07860-9
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2018
• End date: December 31, 2022
• Field of knowledge: Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
• Principal Investigator: Luiz Henrique Capparelli Mattoso
• Grantee: Marcela Piassi Bernardo
• Host Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). São Carlos , SP, Brazil
• Associated research grant: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis, AP.TEM
• Associated scholarship(s): 19/23027-8 - Development of 3D printed scaffolds for bone reconstruction and evaluation of interaction through cellular study, BE.EP.PD

Abstract

There is a growing number of bone injury cases worldwide requiring effective surgical procedures. Biomaterial nanocomposites are promising materials for bone tissue engineering due to the possibility of allying, through the appropriate choice of a polymer matrix and inorganic filler, biocompatibility, bioabsorption and mechanical resistance properties to be compatible with bone structures. Recently, rapid prototyping techniques (3D printing) have become popular, making possible to fabricate bone implant structures with complex shapes at a suitable scale. One of the main techniques with equipment already available in the national market is that based on polymer filaments melting. However, pure polymer filaments are limited due to the lack of functional, biological properties. This project has as main objective the development of functional osteointegratable nanocomposite filaments suitable for melt 3D printing, which should hold adequate mechanical properties, aiming at tissue engineering and regenerative medicine applications. These nanocomposites filaments will be produced from PLA (poly(lactic acid), biocompatible and biodegradable polymer), nanohydroxyapatite (inorganic phase necessary for bone tissue regeneration) and anti-inflammatory drug-intercalated layered double hydroxides (naproxen). The nanocomposites will be obtained by extrusion and detailed studies on the interaction between these constituents, seeking parameters optimization for 3D printing, will be conducted. Characterizations will be performed by XRD, FTIR, TG / DTG, SEM, TEM, mechanical tests (tensile strength, compression, shear and torsion), evaluation of cell differentiation, cytotoxicity, biocompatibility, immune response (in vitro assays) and drug release studies. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLDONI, FERNANDA C. A.; BERNARDO, MARCELA P.; FILHO, JOSEMAR G. OLIVEIRA; DE AGUIAR, ALINE C.; MOREIRA, FRANCYS K. V.; MATTOSO, LUIZ H. C.; COLNAGO, LUIZ A.; FERREIRA, MARCOS D.. Valorization of mangoes with internal breakdown through the production of edible films by continuous solution casting. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 145, JUN 2021. (18/07860-9, 19/13656-8, 18/24612-9)

MALAFATTI, JOAO O. D.; BERNARDO, MARCELA P.; MOREIRA, FRANCYS K. V.; CIOL, HELOISA; INADA, NATALIA M.; MATTOSO, LUIZ H. C.; PARIS, ELAINE C.. Electrospun poly(lactic acid) nanofibers loaded with silver sulfadiazine/[Mg-Al]-layered double hydroxide as an antimicrobial wound dressing. POLYMERS FOR ADVANCED TECHNOLOGIES, v. 31, n. 6, p. 1377-1387, JUN 2020. (13/07276-1, 18/07860-9)

BERNARDO, M. P.; FERREIRA, F. V.; OLIVEIRA, L. F.; MATTOSO, L. H. C.; LOPES, J. H.. Revolutionizing bone regeneration: 3D printing of PLA/MFBG composites with advanced healing properties. MATERIALS TODAY CHEMISTRY, v. 43, p. 13-pg., 2024-12-20. (23/07910-4, 18/07860-9, 20/07956-6, 22/03247-6)

BERNARDO, MARCELA PIASSI; RODRIGUES DA SILVA, BRUNA CRISTINA; CAPPARELLI MATTOSO, LUIZ HENRIQUE. Development of three-dimensional printing filaments based on poly(lactic acid)/hydroxyapatite composites with potential for tissue engineering. JOURNAL OF COMPOSITE MATERIALS, v. 55, n. 17, p. 2289-2300, JUL 2021. (18/07860-9)

MUNHOZ, DAVI R.; BERNARDO, MARCELA P.; MALAFATTI, JOAO O. D.; MOREIRA, FRANCYS K. V.; MATTOSO, LUIZ H. C.. Alginate films functionalized with silver sulfadiazine-loaded [Mg-Al] layered double hydroxide as antimicrobial wound dressing. International Journal of Biological Macromolecules, v. 141, p. 504-510, DEC 1 2019. (10/11584-5, 12/21867-0, 18/07860-9)

BERNARDO, MARCELA P.; RODRIGUES, BRUNA C. S.; DE OLIVEIRA, TAMIRES D.; GUEDES, ADRIANA P. M.; BATISTA, ALZIR A.; MATTOSO, LUIZ H. C.. NAPROXEN/LAYERED DOUBLE HYDROXIDE COMPOSITES FOR TISSUE-ENGINEERING APPLICATIONS: PHYSICOCHEMICAL CHARACTERIZATION AND BIOLOGICAL EVALUATION. CLAYS AND CLAY MINERALS, v. 68, n. 6, JAN 2021. (18/07860-9)

BLANCO, GUILHERME E. DE O.; DE SOUZA, CLOVIS W. O.; BERNARDO, MARCELA P.; ZENKE, MARTIN; MATTOSO, LUIZ H. C.; MOREIRA, FRANCYS K. V.. Antimicrobially active gelatin/[Mg-Al-CO3 ]-LDH composite films based on clove essential oil for skin wound healing. MATERIALS TODAY COMMUNICATIONS, v. 27, JUN 2021. (19/23027-8, 18/07860-9)

BERNARDO, MARCELA P.; DA SILVA, BRUNA C. R.; HAMOUDA, I, AHMED E.; DE TOLEDO, MARCELO A. S.; SCHALLA, CARMEN; RUTTEN, STEPHAN; GOETZKE, ROMAN; MATTOSO, LUIZ H. C.; ZENKE, MARTIN; SECHI, ANTONIO. LA/Hydroxyapatite scaffolds exhibit in vitro immunological inertness and promote robust osteogenic differentiation of human mesenchymal stem cells without osteogenic stimul. SCIENTIFIC REPORTS, v. 12, n. 1, FEB 11 2022. (18/07860-9, 19/23027-8)

BERNARDO, MARCELA P.; PASQUINI, DANIEL; MATTOSO, LUIZ H. C.. Enhanced antibacterial activity of wound dressings based on alginate/hydroxyapatite modified with copper and naproxen. Journal of Materials Research, v. 39, n. 5, p. 12-pg., 2023-12-18. (18/07860-9)