Advanced search
Start date
Betweenand

Determination of the major monohydroxylated metabolites of polycyclic aromatic hydrocarbons in urine using volumetric absorptive microsampling and quantification by LC-MS/MS

Grant number: 18/11150-7
Support type:Scholarships in Brazil - Master
Effective date (Start): November 01, 2018
Effective date (End): November 30, 2019
Field of knowledge:Health Sciences - Pharmacy - Toxicological Analysis
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal Investigator:Fernando Barbosa Júnior
Grantee:João Carlos Jacinto da Cunha de Souza
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

The Polycyclic Aromatic Hydrocarbons (PAHs) are persistent organic pollutants, formed in the incomplete combustion of organic matter being ubiquitous in soil, water, and air. These compounds are known to promote the formation of carcinogenic molecules in living organisms, as well as being potential endocrine disrupters. Human biomonitoring (HBM) consists of the periodic measurement of a particular chemical or its metabolite in the blood or urine of a population in order to assess exposure and health risks. This method has become common in developed countries but is still an uncommon practice in Brazil. However, due to the difficulty of collecting, storing and transporting samples, especially in regions without infrastructure and difficult access, alternative collection techniques based on micro-sampling have been proposed. A promising collection method has recently been proposed, the Volumetric Absorptive Microsampling (VAMS). This method offers a number of advantages over conventional collection procedures, mainly by considerably reducing the sample volume collected. However, little is known about the stability of the analytes after deposition of the biological sample in this device, as well as possible contamination of the sample by the solid substrate. In view of the above, the present project aims to develop and validate a method for the determination of the main monohydroxylated metabolites of PAHs in urine samples, using the VAMS technique as a collection method, followed by quantification using liquid chromatography coupled to mass spectrometry. Furthermore, this project will maybe enable your future application in HBM programs in Brazil.