Characterization and in vitro investigations of growth factor-enriched biosilica for bone repair

• Grant number: 18/16328-9
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date until: October 30, 2018
• End date until: October 29, 2019
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Ana Claudia Muniz Renno
• Grantee: Paulo Roberto Gabbai Armelin
• Supervisor: Rui Luis Goncalves dos Reis
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
• Institution abroad: Universidade do Minho (UMinho), Portugal
• Associated to the scholarship: 15/20704-8 - Performance of bioglass/collagen/magnesium composites in bone repair of rats, BP.PD

Abstract

Bone fractures characterize an important event in the medical healthcare, being related to traumas, aging and diseases. In critical conditions, such as extensive bone loss and osteoporosis, the tissue restoration may be compromised and culminate in a non-union consolidation. In this context, the osteogenic properties of biomaterials with a natural origin have gained prominence. Particularly, marine sponges are promising organisms that can be exploited as biomaterials for bone grafts. Toward the improvement of bioactive materials osteogenic performance, as sponges Biosilica (BS), a feasible strategy is the incorporation of bone growth factors into these materials. In this context, bone morphogenetic protein 2 (BMP2) presents key and positive roles in bone formation. Thus, the objectives of this study are to study the physicochemical and morphological properties of BMP2-enriched BS by using scanning electron microscopy, Fourier transform infrared, X-ray diffraction (SEM, FTIR and XRD respectively), mechanical test and in vitro release of BMP2. In addition, tests on an osteoblast precursor cell line (MC3T3-E1) will be performed to investigate the cytotoxicity and influence on cell proliferation and adhesion in presence of BS. Bioglass (BG) will be used as gold standard material for comparison purposes. This proposal may outcome in the development of novel products for biomedical applications, improving life quality of patients impaired by problems with bone repair. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GABBAI-ARMELIN, PAULO ROBERTO; KIDO, HUELITON WILIAN; FERNANDES, KELLY ROSSETTI; FORTULAN, CARLOS ALBERTO; MUNIZ RENNO, ANA CLAUDIA. Effects of bio-inspired bioglass/collagen/magnesium composites on bone repair. JOURNAL OF BIOMATERIALS APPLICATIONS, v. 34, n. 2, p. 261-272, AUG 2019. (18/16328-9, 15/20704-8)