Genomic analysis of the rodent Holochilus sciureus (Cricetidae: Sigmodontinae: Oryzomyini) with emphasis on the interface between B and sex chromosomes

• Grant number: 18/09553-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2018
• End date: September 30, 2022
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Principal Investigator: César Martins
• Grantee: Camila do Nascimento Moreira
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated research grant: 15/16661-1 - Sex and B chromosome enigmas: model systems for the study of chromosome and genome evolution, AP.TEM

Abstract

Holochilus sciureus (HSC) (2n = 56+1B, and FN = 56) belongs to the rodent tribe Oryzomyini, the most taxonomically diverse tribe of the Sigmodontinae subfamily. Cytogenetic studies on the Oryzomyini reflect such diversity revealing an exceptional range of karyotype variability. Comparative cytogenetics by Zoo-FISH on 15 Oryzomyini species using the entire chromosome set of HSC revealed a highly genome reshuffling involving autosomal, sex, and B chromosomes. Hybridization of Bs, X, and Y chromosome probes of HSC on 12 Oryzomyini species revealed that Bs and sex chromosomes share a common heterochromatic region. Also, the diversity of Oryzomyini Bs in number, size, morphology, and genetic content may be explained by the independent origin of these chromosomes in different subgroups of species. Within mammals, Bs are most frequent in rodents, which account for 70% of the mammalian species that bear them. The use of chromosome-specific DNA libraries constructed by microdissection or chromosome flow sorting has allowed detailed analysis of the origin and molecular structure of Bs. Application of next-generation sequencing in the analysis of Bs revealed that they comprise fragments of standard chromosomes and organellar genomes. Therefore, we propose to analyze for the first time the whole genome sequence of HSC females with and without B chromosome, and the chromosomes B1, B2, X, and Y of HSC, isolated by flow sorting, in order to investigate the sequence homology shared between Bs and the sex chromosomes of HSC. In addition, the sequenced genome of HSC will be used to search for repetitive DNA clusters aiming at understanding the role of these elements in the karyotype variability of Oryzomyini species.

Scientific publications

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

MOREIRA, CAMILA DO NASCIMENTO; PERCEQUILLO, ALEXANDRE REIS; FERGUSON-SMITH, MALCOLM ANDREW; YONENAGA-YASSUDA, YATIYO; VENTURA, KAREN. Chromosomal evolution of tribe Oryzomyini (Rodentia: Cricetidae: Sigmodontinae). Mammalian Biology, v. 102, n. 2, p. 24-pg., 2022-03-17. (09/16009-1, 16/20055-2, 18/09553-6)

MOREIRA, CAMILA DO NASCIMENTO; DALAPICOLLA, JERONYMO; YONENAGA-YASSUDA, YATIYO; MARTINS, CESAR; PERCEQUILLO, ALEXANDRE REIS. Cytogenetic data from remote areas of amazon: new karyotypes for spiny rats (Rodentia: Echimyidae). MAMMALIA, v. N/A, p. 7-pg., 2024-11-11. (15/02853-6, 16/20055-2, 18/09553-6, 09/16009-1)

MOREIRA, CAMILA N.; PRICOLI, FERNANDA G.; FERGUSON-SMITH, MALCOLM A.; YONENAGA-YASSUDA, YATIYO; VENTURA, KAREN. Karyotypic Reshuffling in the Genus Rhipidomys (Rodentia: Cricetidae: Sigmodontinae) Revealed by Zoo-FISH. Cytogenetic and Genome Research, v. 164, n. 2, p. 11-pg., 2024-10-01. (18/09553-6)