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Evaluation of gene expression of cardiac myosins in rats with pulmonary hypertension submitted to preventive training

Grant number: 18/12526-0
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2018
Effective date (End): August 31, 2019
Field of knowledge:Health Sciences - Physiotherapy and Occupational Therapy
Principal Investigator:Francis Lopes Pacagnelli
Grantee:Raisa de Jesus Dutra Dias
Host Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil


Pulmonary hypertension (PH) is a serious disease that progresses to right heart failure (HF) and one of the main causes is chronic obstructive pulmonary disease. The right HF is related to functional limitations and high rates of morbidity and mortality and the exercise in a preventive manner, could be beneficial in cardiac disorders. Objective: to evaluate the preventive physical training in rats with right ventricular dysfunction in relation a gene expression of cardiac myosin. Methods: 32 male Wistar rats will be used, divided into 4 groups (n = 8): sedentary control; training control; sedentary monocrotaline; training monocrotaline. The preventive training protocol will be performed on a treadmill for 13 weeks, 5 times/week, with a 2 week adaptation period and gradual increases in speed/time. In the third, fourth and fifth weeks, the training will be performed at 0.9km/h-60min, in the sixth, seventh and eighth weeks at 1km/h-60min and in the ninth and tenth weeks at 1.1km/h-60min.Next, the monocrotaline (60mg/Kg) will be administered to induce pulmonary arterial hypertension and the lactate threshold analyzed to determine the training speeds for the following 3 weeks. After identification of ventricular dysfunction through echocardiography, the animals will be euthanized. The myosin cadiac gene will analysis using gene expression (RT-qPCR). Normality of the data will be analyzed using the Shapiro-Wilk test and data comparisons using the ANOVA with Tukey's or Dunn's post test (p <5%).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MARIANO, THAOAN BRUNO; DE SOUZA CASTILHO, ANTHONY CESAR; DIAS DE ALMEIDA SABELA, ANA KARENINA; DE OLIVEIRA, ANDRE CASANOVA; CURY, SARAH SANTILONI; AGUIAR, ANDREO FERNANDO; DIAS, RAISA DE JESUS DUTRA; CICOGNA, ANTONIO CARLOS; OKOSHI, KATASHI; JUSTULIN JUNIOR, LUIS ANTONIO; et al. reventive training does not interfere with mRNA-encoding myosin and collagen expression during pulmonary arterial hypertensio. PLoS One, v. 16, n. 9, . (18/12526-0, 18/24317-7)

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