Evaluation of gene expression of cardiac myosins in rats with pulmonary hypertension submitted to preventive training

• Grant number: 18/12526-0
• Support type: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): September 01, 2018
• Effective date (End): August 31, 2019
• Field of knowledge: Health Sciences - Physiotherapy and Occupational Therapy
• Principal researcher: Francis Lopes Pacagnelli
• Grantee: Raisa de Jesus Dutra Dias
• Home Institution: Pró-Reitoria de Pesquisa e Pós-Graduação. Universidade do Oeste Paulista (UNOESTE). Presidente Prudente , SP, Brazil

Abstract

Pulmonary hypertension (PH) is a serious disease that progresses to right heart failure (HF) and one of the main causes is chronic obstructive pulmonary disease. The right HF is related to functional limitations and high rates of morbidity and mortality and the exercise in a preventive manner, could be beneficial in cardiac disorders. Objective: to evaluate the preventive physical training in rats with right ventricular dysfunction in relation a gene expression of cardiac myosin. Methods: 32 male Wistar rats will be used, divided into 4 groups (n = 8): sedentary control; training control; sedentary monocrotaline; training monocrotaline. The preventive training protocol will be performed on a treadmill for 13 weeks, 5 times/week, with a 2 week adaptation period and gradual increases in speed/time. In the third, fourth and fifth weeks, the training will be performed at 0.9km/h-60min, in the sixth, seventh and eighth weeks at 1km/h-60min and in the ninth and tenth weeks at 1.1km/h-60min.Next, the monocrotaline (60mg/Kg) will be administered to induce pulmonary arterial hypertension and the lactate threshold analyzed to determine the training speeds for the following 3 weeks. After identification of ventricular dysfunction through echocardiography, the animals will be euthanized. The myosin cadiac gene will analysis using gene expression (RT-qPCR). Normality of the data will be analyzed using the Shapiro-Wilk test and data comparisons using the ANOVA with Tukey's or Dunn's post test (p <5%).