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Integrated molecular approaches to elucidate the ecological associations of Triatoma brasiliensis: food sources, microbiota and diversity of Trypanosoma cruzi

Grant number: 17/21359-8
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): November 01, 2018
Effective date (End): April 30, 2020
Field of knowledge:Biological Sciences - Parasitology
Principal Investigator:Carlos Eduardo de Almeida
Grantee:Maurício Lilioso de Lucena Filho
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:16/08176-9 - An integrative approach to morphological and molecular diversity of Triatoma brasiliensis, the main Chagas Disease vector in the Brazilian semiarid: elucidating links along the epidemiological chain, AP.JP

Abstract

In the semi-arid zones of Brazil, Triatoma brasiliensis is the most important vector of Chagas' disease. This triatomine is involved in a complex eco-epidemiological net because it occupies different environments, such as wild, peri-domiciliary and domiciliary. Before the advent of new generation sequencing, the molecular techniques available to access the ecological associations of triatomines had limitations for recognizing the diversity of the organisms associated to the vector. Therefore, this project aims to apply an integrative molecular approach via metabarcoding to (i) characterize the food sources of T. brasiliensis; (ii) recognize the intestinal bacterial microbiota of this vector and (iii) the diversity of Trypanosoma cruzi carried by T. brasiliensis. The DNA extracted from the digestive tract of insect populations from the three environments where the species can be found in the state of Rio Grande do Norte will be used. For the hosts recognition and the bacterial community of the intestinal microbiota the 16S rRNA gene sequences present in the gut of the insects with specific primers for each group will be analyzed. To recognize the diversity of T. cruzi, a multiplex PCR with three primers for the region of the mini-exon of this parasite will be performed. The protocol using metabarcoding exhibits greater sensitivity compared to conventional molecular approaches to detect, for example, multiple blood feedings in insects as well as multiclonal T. cruzi infections. The integrated analysis of the results may allow the evaluation of ecological interactions that combine to modulate the parasite transmission. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CHABOLI ALEVI, KAIO CESAR; BITTINELLI, ISADORA DE FREITAS; GRZYB DELGADO, LUIZA MARIA; MADEIRA, FERNANDA FERNANDEZ; DE OLIVEIRA, JADER; LILIOSO, MAURICIO; FOLLY-RAMOS, ELAINE; DA ROSA, JOAO ARISTEU; VILELA AZEREDO-OLIVEIRA, MARIA TERCILIA. Molecular cytotaxonomy of the Triatoma brasiliensis species subcomplex (Hemiptera, Triatominae). Acta Tropica, v. 201, JAN 2020. Web of Science Citations: 0.
LIMA-OLIVEIRA, TARCIANNE M.; FONTES, FERNANDA VON HERTWIG M.; LILIOSO, MAURICIO; PIRES-SILVA, DAYANE; GERALDES TEIXEIRA, MARTA MARIA; VERGARA MEZA, JOSE GABRIEL; HARRY, MYRIAM; FILEE, JONATHAN; COSTA, JANE; VALENCA-BARBOSA, CAROLINA; FOLLY-RAMOS, ELAINE; ALMEIDA, CARLOS EDUARDO. Molecular eco-epidemiology on the sympatric Chagas disease vectors Triatoma brasiliensis and Triatoma petrocchiae: Ecotopes, genetic variation, natural infection prevalence by trypanosomatids and parasite genotyping. Acta Tropica, v. 201, JAN 2020. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.