Shell structural alterations and biochemical responses in limpets as biomarkers of environmental contamination

• Grant number: 18/08015-0
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date until: October 01, 2018
• End date until: December 31, 2021
• Field of knowledge: Physical Sciences and Mathematics - Oceanography - Chemical Oceanography
• Principal Investigator: Ítalo Braga de Castro
• Grantee: Cyntia Ayumi Yokota Harayashiki
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
• Associated scholarship(s): 20/14576-5 - Shell characterization of three mollusk species under different degrees of environmental contamination, BE.EP.PD

Abstract

Coastal aquatic environments can be impacted due to different anthropogenic activities. As consequence, organisms inhabiting these areas are frequently exposed to a mixture of hazardous chemical substances. Recently, studies in South-American coastal zones showed that limpets collected in environments with different degrees of contamination presented shells with morphological alterations, which indicates that these alterations can be potentially used as biomarkers of contamination. However, few experimental and temporal studies have been undertaken so far in order to globally validate this tool in monitoring studies. This project proposal intends to investigate the relationship between morphological and structural parameters from Lottia subrugosa shells and biochemical responses in soft tissues of these organisms under experimental and temporal contamination scenarios. In the experimental study, organisms from unpolluted sites will be transplanted in highly contaminated areas previously identified in the littoral of São Paulo (Brazil). The temporal assessment will compare shells deposited in zoological collections and shells from organisms that will be collected for this project. The results from this study will allow the validation of these tools as biomarkers of environmental contamination for coastal zones. Additionally, considering that protein in shells can be preserved for up to a million years, the temporal approach might assist in temporal reconstruction of environmental toxicity. Finally, the implementation of this proposal will allow the establishment of a doctor recently degreed at Southern Cross University (Australia) with expertise in ecotoxicology field in an institution in the State of São Paulo.

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

YOKOTA HARAYASHIKI, CYNTIA AYUMI; MARQUEZ, FEDERICO; CARIOU, ELSA; CASTRO, ITALO BRAGA. Mollusk shell alterations resulting from coastal contamination and other environmental factors. Environmental Pollution, v. 265, n. B, OCT 2020. (15/25063-0, 16/50409-0, 18/08015-0)

RIBEIRO, VICTOR VASQUES; YOKOTA HARAYASHIKI, CYNTIA AYUMI; ERTAS, ALPEREN; CASTRO, ITALO BRAGA. Anthropogenic litter composition and distribution along a chemical contamination gradient at Santos Estuarine System-Brazil. REGIONAL STUDIES IN MARINE SCIENCE, v. 46, JUL 2021. (19/13750-4, 18/08015-0)

YOKOTA HARAYASHIKI, CYNTIA AYUMI; SADAUSKAS-HENRIQUE, HELEN; DE SOUZA-BASTOS, LUCIANA RODRIGUES; GOUVEIA, NAYARA; LUNA, ANA JULYA; OSTRENSKY, ANTONIO; CASTRO, ITALO BRAGA. Contamination gradient affects differently carbonic anhydrase activity of mollusks depending on their feeding habits. ECOTOXICOLOGY, v. 31, n. 1, NOV 2021. (18/08015-0, 19/23310-1, 15/25063-0)

HARAYASHIKI, CYNTIA AYUMI YOKOTA; SADAUSKAS-HENRIQUE, HELEN; SOUZA-BASTOS, LUCIANA RODRIGUES DE; GOUVEIA, NAYARA; PONT, GIORGI DAL; OSTRENSKY, ANTONIO; CASTRO, ITALO BRAGA. Shell form and enzymatic alterations in Lottia subrugosa (Gastropoda, Lotiidae) transplanted to a contaminated site. Marine Pollution Bulletin, v. 164, MAR 2021. (18/08015-0)

GOUVEIA, NAYARA; YOKOTA HARAYASHIKI, CYNTIA AYUMI; MARQUEZ, FEDERICO; LOURENCO, RAFAEL ANDRE; TANIGUCHI, SATIE; CASTRO, ITALO BRAGA. Mollusc shell shape as pollution biomarkers: Which is the best biological model. Marine Pollution Bulletin, v. 179, p. 10-pg., 2022-04-21. (18/08015-0, 16/18348-1, 15/25063-0)

BRAGA, ANNA RAFAELA CAVALCANTE; DE ROSSO, VERIDIANA VERA; HARAYASHIKI, CYNTIA AYUMI YOKOTA; JIMENEZ, PAULA CHRISTINE; CASTRO, ITALO BRAGA. Global health risks from pesticide use in Brazil. NATURE FOOD, v. 1, n. 6, p. 3-pg., 2020-06-01. (18/08015-0)

YOKOTA HARAYASHIKI, CYNTIA AYUMI; MARQUEZ, FEDERICO; CARIOU, ELSA; CASTRO, ITALO BRAGA. Mollusk shell alterations resulting from coastal contamination and other environmental factors. Environmental Pollution, v. 265, p. 13-pg., 2020-10-01. (15/25063-0, 16/50409-0, 18/08015-0)

YOKOTA HARAYASHIKI, CYNTIA AYUMI; MARTINS, CAMILA PRATALLI; MARQUEZ, FEDERICO; BIGATTI, GREGORIO; CASTRO, ITALO BRAGA. Historical shell form variation in Lottia subrugosa from southeast Brazilian coast: Possible responses to anthropogenic pressures. Marine Pollution Bulletin, v. 155, JUN 2020. (15/25063-0, 16/50409-0, 18/08015-0)

GOUVEIA, NAYARA; OLIVEIRA, ANA JULYA L. A.; YOKOTA HARAYASHIKI, CYNTIA AYUMI; SOUZA, JOSIANE CARNEIRO; LONGO, ELSON; CANO, NILO F.; MALTEZ, HELOISA FRANCA; LOURENCO, RAFAEL ANDRE; TURPO-HUAHUASONCCO, KLINTON, V; CASTRO, ITALO BRAGA. Chemical contamination in coastal areas alters shape, resistance and composition of carnivorous gastropod shells. Chemosphere, v. 307, p. 10-pg., 2022-08-06. (18/08015-0, 13/07296-2, 15/25063-0)