Localization of senescent cells in lesions from patients with deep Endometriosis

• Grant number: 18/11042-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2018
• End date: March 31, 2022
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Sérgio Podgaec
• Grantee: Helena Malvezzi
• Host Institution: Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE). São Paulo , SP, Brazil

Abstract

Endometriosis is characterized by implantation and growth of endometrial tissue outside the uterine cavity. It's pathology can be sustained by genetic predisposition, hormonal changes, progesterone resistance, estrogen dependence, inflammation, angiogenesis and vascularigenesis, oxidative stress, and immunological factors. There seems to be a similarity between Endometriosis and Cancer, as both are inflammatory diseases, with high vascularity, angiogenesis and produce higher Reactive Oxygen Species (ROS). It is worth noting the presence of biomarkers for senescent cells in several types of Cancer aside from accelerated cell proliferation followed by a sudden drop in growth when the presence of senescent cells are noticed in lung Cancer and melanomas in animal models. Cell senescence is an irreversible process suspended cell proliferation, which is responsible for controlling unrestrained growth as well as a cell repair pathways. Since Endometriosis is a disease characterized by the presence of angiogenesis, immunological and inflammatory factors and high concentrations of ROS, as well as in Cancer, we hypothesize that there are senescent cells present in the lesion and in the endometrium and that oxidative stress and ROS-induced pathways induce senescence in the cells of these sites helping the maintenance of the disease. In this project, we will use biopsies, peritoneal fluid and cell culture to test our hypothesis. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PALMIERI, LAURA; MALVEZZI, HELENA; DE AZEVEDO, BRUNA CESTARI; PLACENCIA, EDUARDO VAREJAO DIAZ; ANTONIOLI, ELIANE; PODGAEC, SERGIO. High levels of a comprehensive set of matrix metalloproteinases in endometriotic lesions: validating the key role of cellular senescence in endometriosis pathogenesis. Einstein (São Paulo), v. 23, p. 10-pg., 2025-01-01. (18/11042-0, 22/10279-1)

DA COSTA, KATIANE DE ALMEIDA; MALVEZZI, HELENA; DOBO, CRISTINE; NEME, ROSA MARIA; FILIPPI, RENEE ZON; ARRAIS ALOIA, THIAGO PINHEIRO; PRADO, ELISA RAMPAZO; MEOLA, JULIANA; PICCINATO, CARLA DE AZEVEDO. Site-Specific Regulation of Sulfatase and Aromatase Pathways for Estrogen Production in Endometriosis. FRONTIERS IN MOLECULAR BIOSCIENCES, v. 9, p. 13-pg., 2022-05-03. (18/11042-0)

PALMIERI, LAURA; MALVEZZI, HELENA; CESTARI, BRUNA; PODGAEC, SERGIO. Colocalization of senescent biomarkers in deep, superficial, and ovarian endometriotic lesions: a pilot study. SCIENTIFIC REPORTS, v. 12, n. 1, p. 14-pg., 2022-10-14. (18/11042-0)

MALVEZZI, HELENA; HERNANDES, CAMILA; DE AZEVEDO, BRUNA C.; PICCINATO, CARLA A.; MEOLA, JULIANA; PODGAEC, SERGIO. INCREASED CELLULAR SENESCENCE IN DEEP INFILTRATING ENDOMETRIOSIS.. Fertility and Sterility, v. 114, n. 3, p. 1-pg., 2020-09-01. (18/11042-0)

MALVEZZI, HELENA; DOBO, CRISTINE; FILIPPI, RENEE ZON; DO NASCIMENTO, HELEN MENDES; DA SILVA E SOUSA, LAURA PALMIERI; MEOLA, JULIANA; PICCINATO, CARLA AZEVEDO; PODGAEC, SERGIO. Altered p16(Ink4a), IL-1 beta, and Lamin b1 Protein Expression Suggest Cellular Senescence in Deep Endometriotic Lesions. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, n. 5, p. 15-pg., 2022-03-01. (18/11042-0)

MALVEZZI, HELENA; HERNANDES, CAMILA; PICCINATO, CARLA A.; PODGAEC, SERGIO. Interleukin in endometriosis-associated infertility-pelvic pain: systematic review and meta-analysis. Reproduction, v. 158, n. 1, p. 1-12, JUL 2019. (18/11042-0)

ANNICCHINO, GIULIANA; MALVEZZI, HELENA; PICCINATO, CARLA DE AZEVEDO; PODGAEC, SERGIO. Existe um risco maior para desfechos obstétricos desfavoráveis em mulheres com endometriose? Uma avaliação das evidências. Revista Brasileira de Ginecologia e Obstetrícia, v. 42, n. 4, p. 200-210, APR 2020. (18/11042-0)

MALVEZZI, HELENA; MARENGO, ELIANA BLINI; PODGAEC, SERGIO; PICCINATO, CARLA DE AZEVEDO. Endometriosis: current challenges in modeling a multifactorial disease of unknown etiology. JOURNAL OF TRANSLATIONAL MEDICINE, v. 18, n. 1, AUG 12 2020. (18/11042-0)