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Quantification of microbial populations and ruminanl fermentation of Nellore cattle fed with different levels of wet corn distillers grains

Grant number: 18/09731-1
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): November 01, 2018
Effective date (End): July 14, 2021
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Principal Investigator:Mário de Beni Arrigoni
Grantee:Pablo de Souza Castagnino
Host Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated scholarship(s):19/19645-8 - Microbial biodiversity analysis and utilization of branched chain volatile fatty acids by rumen bacteria, BE.EP.PD


The interactions between the rumen microbial populations can be changed by nutritional strategies adopted and it can affect the fermentation dynamics and consequently the beef cattle performance . The use of co-products in the diets of feedlot beef cattle has increased to reduce feed costs. A new co-product are the distillery grains from the corn based ethanol industry. The information about the changes of the fermentation and the ruminal microbial diversity of the high concentrate diets of Nellore cattle fed with wet corn distillers grains is less clear and the knowledge of this information is essential for the formulation of diets that modulate the ruminal fermentation and potentiate the animal performance. The objective of this study will be to evaluate the effects of inclusion of levels of wet corn distillers grains (WDG) on the proportions of ruminal microbial populations and the fermentation of Nellore cattle. Eight rumen cannulated Nellore cattle (BW= 329) will be used in a replicated 4 × 4 Latin square design. The animals will be distributed in 4 treatments: T1: 0%; T2: 15%; T3: 30% and T4: 45% WDG in dry matter (DM). Experimental diets will be formulated with ground corn, soybean meal, mineral nucleus and tifton 85 hay. The experiment will be conducted in four 21-day periods with 14 days of adaptation to the experimental diet and the last 7 days used for the quantification of microbial populations using the real-time PCR technique and other fermentative parameters. The data will be analyzed by orthogonal contrasts by the PROC MIXED procedure of the statistical software SAS (SAS Inst. Inc., Cary, NC).

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TOMAZ, LAIS DE AQUINO; NIEHUES, MARIA BETANIA; BALDASSINI, WELDER ANGELO; CASTAGNINO, PABLO; AMARAL BERTOCO, JOAO PEDRO; VAN CLEEF, ERIC; EZEQUIEL, JANE; ARRIGONI, MARIO DE BENI; MARTINS, CYNTIA L.; NETO, OTAVIO R. MACHADO. Increasing levels of de-oiled wet distillers grains in feedlot diets on nutrient digestibility, ruminal fermentation and microbiology. LIVESTOCK SCIENCE, v. 245, . (17/21354-6, 18/09731-1, 16/04478-0)

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