RED BLOOD CELL ALLOIMMUNIZATION IN SICKLE CELL DISEASE: IDENTIFICATION OF RH VARIANTS AND IMMUNE MARKERS

• Grant number: 17/26950-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: November 01, 2018
• End date: October 31, 2021
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Simone Kashima Haddad
• Grantee: Evandra Strazza Rodrigues Sandoval
• Host Institution: Hemocentro de Ribeirão Preto. Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da USP (HCMRP). Secretaria da Saúde (São Paulo - Estado). Ribeirão Preto , SP, Brazil

Abstract

Red blood cell alloimmunization in sickle cell disease (SCD) affects more than 30% of individuals in transfusion therapy. It is the main complication related to blood transfusion in SCD and may be related to the antigenic disparity between donors and patients or with a deficiency in the control of the cellular immune response. The prophylaxis adopted to prevent alloimmunization is to supply red blood cell units compatible with the most immunogenic antigens; however, the occurrence of RHD/ RHCE gene variants often makes this strategy ineffective. As an alternative, some centers use genotyping, notwithstanding, most of the molecular assays used are unable to detect new mutations, predict null alleles, or even massively screening donors and patients quickly.Regarding the immunological profile, alloimmunized SCD patients present a quantitative and functional alteration in regulatory and effector T lymphocytes. Consequently, the production of antibodies and the synthesis of inflammatory cytokines may also exhibit altered activities. However, the timing and motive that makes immunological cellular activity pathogenic in these individuals are unknown. Thus, to understand the stages of red blood cell alloimmunization process in SCD patients, this proposal aims to evaluate the frequency, function of T cell population and profile of inflammatory cytokines. In addition, RHD/ RHCE gene variants will also be determined in red blood cell alloimmunized SCD patients by next generation sequencing.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SLAVOV, SVETOSLAV N.; PATANE, JOSE S. L.; BEZERRA, RAFAEL DOS SANTOS; GIOVANETTI, MARTA; FONSECA, VAGNER; MARTINS, ANTONIO J.; VIALA, VINCENT L.; RODRIGUES, EVANDRA S.; SANTOS, ELAINE V.; BARROS, CLAUDIA R. S.; et al. Genomic monitoring unveil the early detection of the SARS-CoV-2 B.1.351 (beta) variant (20H/501Y.V2) in Brazil. Journal of Medical Virology, v. 93, n. 12, JUL 2021. (20/10127-1, 18/15826-5, 13/08135-2, 20/05367-3, 20/00694-6, 17/26950-6)

SLAVOV, SVETOSLAV NANEV; GIOVANETTI, MARTA; BEZERRA, RAFAEL DOS SANTOS; FONSECA, VAGNER; SANTOS, ELAINE VIEIRA; RODRIGUES, EVANDRA STRAZZA; ADELINO, TALITA; XAVIER, JOILSON; BORGES, JOSIANE SERRANO; EVARISTO, MARIANE; et al. Molecular surveillance of the on-going SARS-COV-2 epidemic in Ribeirao Preto City, Brazil. INFECTION GENETICS AND EVOLUTION, v. 93, SEP 2021. (13/08135-2, 17/26950-6, 18/15826-5)

RODRIGUES, EVANDRA STRAZZA; ROMAGNOLI, ALINE CRISTINA; SANTOS, FLAVIA LEITE SOUZA; CUTTER, TALITHA BALDIN; CATELLI, LUCAS FERIOLI; CEDRIC, VRIGNAUD; PEYRARD, THIERRY; COVAS, DIMAS TADEU; DE CASTILHO, LILIAN MARIA; KASHIMA, SIMONE. Frequency and characterization of RHD variant alleles in a population of blood donors from southeastern Brazil: Comparison with other populations. TRANSFUSION AND APHERESIS SCIENCE, v. 60, n. 4, AUG 2021. (13/08135-2, 17/26950-6)

SLAVOV, SVETOSLAV NANEV; BEZERRA, RAFAEL DOS SANTOS; RODRIGUES, EVANDRA STRAZZA; SANTOS, ELAINE VIEIRA; BORGES, JOSEANE SERRANO; DE LA ROQUE, DEBORA GLENDA LIMA; PATAN, JOSE SALVATORE LEISTER; LIMA, ALEX RANIERI JERONIMO; RIBEIRO, GABRIELA; VIALA, VINCENT LOUIS; et al. Genomic monitoring of the SARS-CoV-2 B1.1.7 (WHO VOC Alpha) in the Sao Paulo state, Brazil. VIRUS RESEARCH, v. 308, p. 6-pg., 2022-01-15. (20/05367-3, 17/26950-6, 18/15826-5, 19/08528-0)

SLAVOV, SVETOSLAV NANEV; LIMA DE LA-ROQUE, DEBORA GLENDA; MENDES DA COSTA, PERICLES NATAN; RODRIGUES, EVANDRA STRAZZA; SANTOS, ELAINE VIEIRA; BORGES, JOSIANE SERRANO; EVARISTO, MARIANE; MACONETTO, JULIANA DE MATOS; MARQUES, ADRIANA APARECIDA; MILHOMENS, JONATHAN; et al. Dynamics of SARS-CoV-2 Variants of Concern in Vaccination Model City in the State of Sao Paulo, Brazil. Viruses-Basel, v. 14, n. 10, p. 12-pg., 2022-10-01. (14/50947-7, 18/15826-5, 20/10127-1, 20/06441-2, 19/22155-2, 17/26950-6, 21/11944-6, 13/08135-2)