In the initial post-doctoral project proposal submitted to FAPESP, was aimed to characterize the transcriptome and to perform a population genomic study of one of the largest predators in the marine environment, with a broad geographical distribution, and ecological and commercial interest, the mako shark (Isurus oxyrinchus). In addition, the initial proposal aimed at training human resources in the field of bioinformatics and genomics. The laboratorial step, using new generation sequencing techniques - NGS (RNA-seq and ddRADseq), yielded massive amounts of sequencing with more than 42 784 019 million sequences of RNA-seq for the characterization of the transcriptome, and 119 121 972 million sequences of ddRADseq for population genomic study. Thus, the second stage of this project involves the treatment of these data through bioinformatics techniques for subsequent analyzes that may to shed light on biological and ecological significance to the mako shark. Therefore, this project aims: (i) training in bioinformatics to treat sequencing of RNA-seq and ddRADseq, (ii) to identify genes and their associations with biological, physiological and molecular functions, (iii) to select genes that allow inferences on local adaptation as well as an extensive population genomic study in two large oceanic basins, Atlantic and Indian Oceans. At the end of this period abroad, it is expected to obtain new results for this species, which will result in publications in high impact scientific journals, as well as a solid formation of the candidate in genomics and bioinformatics, and collaborations with renowned researchers from abroad who have worked in the frontier of knowledge in this area of research.
News published in Agência FAPESP Newsletter about the scholarship: