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Exploiting the relevance of the protein corona on the cellular uptake of polymeric micelles and polymersomes

Grant number: 18/21272-2
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): February 01, 2019
Effective date (End): July 31, 2019
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
Principal researcher:Fernando Carlos Giacomelli
Grantee:Fernando Augusto de Oliveira
Supervisor abroad: Giuseppe Battaglia
Home Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
Research place: University College London (UCL), England  
Associated to the scholarship:17/19079-7 - Probing the nanobiointerface: dynamic, structure and binding ratio of plasma proteins adsorbed onto polymeric micelles and polymersomes, BP.MS

Abstract

The project aims to understand the relevance of the protein-corona on the cellular uptake of polymeric micelles and polymersomes. Block copolymer micelles and polymersomes will be produced form novel synthesized Cy7-conjugated PHPMA35-b-PDPA35 block copolymers. The presence of the fluorescent dye DBCO-Cyanine 7 (Cy7) chemically conjugated to the polymer chains will enable the evaluation of cellular uptake by confocal laser scanning microscopy (CLSM) and flow cytometry. The self-assemblies will be manufactured by thin-film hydration (polymersomes) or nanoprecipitation (micelles) with further characterization via dynamic (DLS) and electrophoretic (ELS) light scattering, besides transmission electron microscopy (TEM). The Cy7-conjugated PHPMA35-b-PDPA65 assemblies will be exposed to human serum environment in order to enable plasma protein adsorption with further separation and semi-quantification by SDS-PAGE. In the step further, the cellular uptake behavior of protein-free and protein-bound assemblies will be monitored by CLSM and flow cytometry. Overall, we wish to understand the influence of the morphology in the development of protein layers onto the surface of micelles and polymersomes and its relevance to the cellular uptake of supramolecular self-assemblies having a PHPMA stabilizing shell. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE OLIVEIRA, FERNANDO A.; ALBUQUERQUE, LINDOMAR J. C.; RISKE, KARIN A.; JAGER, ELIEZER; GIACOMELLI, FERNANDO C. Outstanding protein-repellent feature of soft nanoparticles based on poly(N-(2-hydroxypropyl) methacrylamide) outer shells. Journal of Colloid and Interface Science, v. 574, p. 260-271, AUG 15 2020. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.