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Evaluation of anatomical damage of CIRCUMJASCENT tissues to the knee of dogs after osteotomy for leveling tibial plateau (TPLO), with or without the use of COMPRESSE, specific surgical FASTENER and modification of osteotomy technique. ex-vivo study

Grant number: 17/25989-6
Support type:Scholarships in Brazil - Master
Effective date (Start): December 01, 2018
Effective date (End): February 29, 2020
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Luis Gustavo Gosuen Gonçalves Dias
Grantee:Rodrigo Casarin Costa
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

Cranial cruciate ligament's feilure in dogs is the main cause of pelvic limb lamness. One of the most used therapeutic options nowadays is the Tibial Plateau Leveling Osteotomy (TPLO) that results in a modification of the knee's biomechanics by angular corrective osteotomy. Due to reports of serious intraoperative complications such as popliteal artery laceration and others possibly underdiagnosed as neurologic damage of the fibular nerve, it is proposed to evaluate the damage of adjacent structures (lateral aspect) to osteotomy and the efficacy of preservation's methods. In this study 5 experimental groups composed of 5 canine corpses with body weight between 20 and 30kg previously prepared with fixation and anatomical conservation solution and later with a barium and latex solution for radiographic detection of the vessels and also it's anatomical avaluation. The left knee will be standardized for proximal tibial semicircular osteotomy without protection methods and the right knee, standard for use of methods of protection of adjacent tissues prior to osteotomy. In the first group, denominated G1 we will use the compress in the cranial to caudal sense. In G2 the compress will be allocated caudal to cranial sense. In G3, the compress will be applied in the cranial to caudal sense, but will be push through caudally to the tibia until it being visible in the operative field. The special retractor developed by the team for this purpose (to protect soft tissues) will be used in groups G4 and G5. In G4, the retractor will be used in the cranial to caudal direction and in G5, in the caudal to cranial direction.In G6, the osteotomy with semicircular oscilatory saw will be performedThe evaluations will be parcial performed at the trans cortex, and be completed after a bending force be applied. by radiographic comparison before and after osteotomy and by dissection of the structures adjacent to the osteotomy line. Data will be assessed by non-parametric analysis using Kruskal Wallis test or Friedman test, followed by multiple comparisons by the Dunn's test. The results will be presented as medians and total amplitude. The significance level established for all the aforementioned statistical analyzes will be 5%.

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