Determination of pharmaceuticals in wastewater

• Grant number: 18/11700-7
• Support Opportunities: Scholarships in Brazil - Master
• Start date: December 01, 2018
• End date: November 02, 2020
• Field of knowledge: Engineering - Sanitary Engineering - Water Supply and Wastewater Treatment
• Principal Investigator: Paulo Clairmont Feitosa de Lima Gomes
• Grantee: César Augusto Marasco Júnior
• Host Institution: Instituto de Química (IQ). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated research grant: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications, AP.TEM
• Associated scholarship(s): 19/03385-7 - MRM-profiling: a new tool for detecting biomarkers, BE.EP.MS

Abstract

The health appeared as the Brazilian federal government's priority in the middle of the 20 th century. Brazilian population has been increasing gradually, it has moved from rural areas to urban centers, where health and sanitation presented better conditions. Moreover, pharmaceutical industry has also been increasing concomitantly with the population, in order to supply the demand of medicines. These pharmaceuticals are important therapeutic agents, however their residues generated during productive process or present in human and animal excreta are persistent in wastewater. This subject aroused the scientific community attention since toxicological effects were observed in human and aquatic fauna and flora. There are unknown toxicological effects caused by pharmaceutical compounds present in wastewater. Therefore, it is essential to monitor the occurrence of such emerging contaminants in wastewater, even more when the population does not have access to the sewage treatment. In addition, wastewater treatment plants were not designed to remove emerging pollutants such as pharmaceutical compounds. Thus, it is necessary to develop reliable analytical methods to determine the pharmaceuticals in wastewaters samples. The liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) has been the choice technique for detection and quantification of pharmaceuticals compounds present in wastewater samples at concentration level ranging from ¼g L-1 to ng L-1. Wastewater samples are complex requiring sample preparation. Solid-phase extraction (SPE) and/or restricted access media (RAM) are techniques of choice to pre-concentrate and extract these compounds. SPE and/or RAM in online mode is an alternative to reduce crossed contamination, allow analysis automation, and requires few steps minimal sample pre-treatment. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARASCO JUNIOR, CESAR AUGUSTO; DA SILVA, BIANCA FERREIRA; LAMARCA, RAFAELA SILVA; FEITOSA DE LIMA GOMES, PAULO CLAIRMONT. Automated method to determine pharmaceutical compounds in wastewater using on-line solid-phase extraction coupled to LC-MS/MS. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, JUN 2021. (18/11700-7, 16/03369-3, 14/50945-4, 18/22393-8)

EDWARDS, MADISON E.; MARASCO, CESAR A.; SCHOCK, TRACEY B.; SOBREIRA, TIAGO J. P.; FERREIRA, CHRISTINA R.; COOKS, R. GRAHAM. Exploratory analysis using MRM profiling mass spectrometry of a candidate metabolomics sample for testing system suitability. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, v. 468, OCT 2021. (18/11700-7, 19/03385-7)

MARASCO JUNIOR, CESAR A.; SARTORE, DOUGLAS M.; LAMARCA, RAFAELA S.; DA SILVA, BIANCA F.; SANTOS-NETO, ALVARO J.; GOMES, PAULO CLAIRMONT F. DE LIMA. On-line solid-phase extraction of pharmaceutical compounds from wastewater treatment plant samples using restricted access media in column-switching liquid chromatography-tandem mass spectrometry. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1180, AUG 15 2021. (18/22393-8, 18/11700-7, 16/03369-3, 14/50945-4)

MARASCO JUNIOR, CESAR AUGUSTO; DA SILVA, BIANCA FERREIRA; LAMARCA, RAFAELA SILVA; FEITOSA DE LIMA GOMES, PAULO CLAIRMONT. Automated method to determine pharmaceutical compounds in wastewater using on-line solid-phase extraction coupled to LC-MS/MS. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 413, n. 20, SI, p. 5147-5160, AUG 2021. (18/11700-7, 14/50945-4, 18/22393-8, 16/03369-3)

JUNIOR, CESAR A. MARASCO; EDWARDS, MADISON E.; LAMARCA, RAFAELA S.; SOBREIRA, TIAGO J. P.; CATERINO, JEFFREY M.; HAINS, DAVID S.; SCHWADERER, ANDREW L.; GOMES, PAULO CLAIRMONT F. DE LIMA; FERREIRA, CHRISTINA R.. Suspect screening of exogenous compounds using multiple reaction screening (MRM) profiling in human urine samples. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1201, p. 7-pg., 2022-06-30. (18/11700-7, 19/03385-7, 18/22393-8, 14/50945-4)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MARASCO JÚNIOR, César Augusto. Determination of pharmaceuticals as contaminants in wastewater using online sample preparation and LC-MS/MS and application of the MRM-Profiling technique in the evaluation of environmental compounds relevance (Exposome). 2020. Master's Dissertation - Universidade Estadual Paulista (Unesp). Instituto de Química. Araraquara Araraquara.