Evaluation of the anticariogenic, irritant and cytotoxic potential of an alcohol-free mouthwash formulation containing pomegranate peel extract (Punica granatum), sodium trimetaphosphate and fluorine

• Grant number: 18/06582-5
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2018
• End date: October 31, 2022
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Debora Barros Barbosa
• Grantee: Ana Paula Miranda Vieira
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated scholarship(s): 20/12321-0 - Antifungal effect of herbal formulation for mouthwash with pomegranate in mucosas infections caused by Candida albicans and Candida glabrata, BE.EP.DR

Abstract

The present study aims to develop a liquid mouthwash formulation (ETF) containing 3% pomegranate peel extract, 0.2% sodium trimetaphosphate (TMP) and 100 ppm fluorine (F), as well as to evaluate its anticariogenic potential, irritant potential and cytotoxicity. The extract of the pomegranate peel will be obtained by alcoholic means and subjected to pharmacopoeial analysis by colorimetry of Folin Denis and Liquid Chromatography of High Efficiency. After obtaining the pomegranate extract, the ETF will be prepared, characterized and subjected to stability analysis. In situ evaluation of the anticariogenic potential will be performed in blocks of bovine tooth enamel coupled to palatine devices containing 4 enamel blocks that will undergo cariogenic challenge (sucrose 30% 6x / day). Volunteers (n = 12) will use the device for 7 days in 4 experimental phases testing the ETF and control groups. After the experimental period, the concentration of alkali-soluble carbohydrates (EPS), fluoride (F), calcium (Ca) and phosphorus (P) will be determined in the biofilm grown on the enamel block. The percentage of surface hardness loss, the hardness in longitudinal section, F, Ca and P present in the enamel will be determined. The irritation potential of the formulation will be determined by the hen's egg testing on the chorioallantoic membrane in four independent trials and will be compared to LISTERINE® Anticaries Zero Alcohol. The cytotoxicity test will be performed on primary cultures of rat fibroblast cells (L9F29), where cell viability will be assessed by the Alamar Blue assay. For statistical analysis, the most appropriate test for the distribution of data will be used, through Sigmaplot© software for Windows version 12.0, with significance at the 5% level. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERNANDES, GABRIELA LOPES; MIRANDA VIEIRA, ANA PAULA; DANELON, MARCELLE; EMERENCIANO, NAYARA GONCALVES; BERRETTA, ANDRESA APARECIDA; MOREIRA BUSZINSKI, ANDREI FELIPE; HORI, JULIANA ISSA; FERNANDES DE LIMA, MIKHAEL HARUO; DOS REIS, THAILA FERNANDA; DE LIMA, JESSICA APARECIDA; et al. Pomegranate Extract Potentiates the Anti-Demineralizing, Anti-Biofilm, and Anti-Inflammatory Actions of Non-Alcoholic Mouthwash When Associated with Sodium-Fluoride Trimetaphosphate. ANTIBIOTICS-BASEL, v. 11, n. 11, p. 19-pg., 2022-11-01. (18/06582-5)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

VIEIRA, Ana Paula Miranda. Addition of pomegranate peel extract in a mouthwash with polyphosphate-fluoride reduces the tooth enamel demineralization. 2022. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.