Chlamydomonas reinhardtii recombinant strains cultivation kinetic

• Grant number: 18/23036-4
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): December 01, 2018
• Effective date (End): June 30, 2019
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Lívia Seno Ferreira Camargo
• Grantee: Jassiara da Silva Pessoa
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
• Associated research grant: 16/12992-6 - Chlamydomonas reinhardtii as a production platform to obtain biopharmaceuticals of national interest, AP.JP

Abstract

Kinetic analysis from microorganisms cultivation is important for process optimization. In this project, cultures of different recombinant strains of Chlamydomonas reinhardtii microalgae will be carried out in different light intensities, in Erlenmeyer flasks and photobioreactor. For each culture, the concentration of the lipids and total proteins, as well as the accumulation of recombinant proteins (Western Blotting), will be evaluated, as well as cell concentration (mg/L) and cell productivity (mg/L/day) will be calculated.