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Chlamydomonas reinhardtii recombinant strains cultivation kinetic

Grant number: 18/23036-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): December 01, 2018
Effective date (End): June 30, 2019
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Lívia Seno Ferreira Camargo
Grantee:Jassiara da Silva Pessoa
Home Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Ministério da Educação (Brasil). Santo André , SP, Brazil
Associated research grant:16/12992-6 - Chlamydomonas reinhardtii as a production platform to obtain biopharmaceuticals of national interest, AP.JP

Abstract

Kinetic analysis from microorganisms cultivation is important for process optimization. In this project, cultures of different recombinant strains of Chlamydomonas reinhardtii microalgae will be carried out in different light intensities, in Erlenmeyer flasks and photobioreactor. For each culture, the concentration of the lipids and total proteins, as well as the accumulation of recombinant proteins (Western Blotting), will be evaluated, as well as cell concentration (mg/L) and cell productivity (mg/L/day) will be calculated.