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Reconstruction of bacterial regulatory networks in environmental terrestrial samples

Grant number: 18/21133-2
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): January 28, 2019
Effective date (End): December 01, 2019
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Rafael Silva Rocha
Grantee:Lummy Maria Oliveira Monteiro
Supervisor abroad: Peter F Stadler
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Local de pesquisa : Leipzig University, Germany  
Associated to the scholarship:16/19179-9 - Deciphering the architecture/function relationship in complex bacterial promoters through synthetic biology approaches, BP.DR


The sharing of genetic information among microbial communities can be represented by a complex network that interconnects the most diverse information, as for example: metabolites, specific genes, enzymes, substrates and also the transcription factors. For instance, the survival and coexistence of a microbial community could be explained or understood through genes expressed by that community, as well as the metabolic products that these organisms produce and share. Since transcription factors are responsible for regulating organisms lives and consequently a community, they can be used to explain and decipher the behavior of these communities. In this sense, these communities could be studied and understood at a regulatory level. Currently, several studies use metagenomics approaches as a potential to understand and explore the dynamics and diversity of microbial communities. However, the understanding of communities behave at regulation level (i.e., set of bacterial regulators present in this community) remains incomplete since many transcriptional regulators are not yet well characterized. In this context, the objective of this project is to develop a systemic approach to analyze the bacterial regulatory network in terrestrial metagenomes, integrating technologies to decode the distribution, abundance, association and statistical correlations of bacterial transcriptions factors in a global ecological scale using a community systems biology approach. Seeking to understand and decipher the behavior of the biological system through bioinformatics tools, our goal is to recover patterns of transcription factors prevalent in various terrestrial metagenomes. Deciphering the complex regulatory network (metaregulation) of a metagenomic community through transcription factor would be the main goal of this project. These data will contribute to the understanding of the complex inter-species and intra-populations relation of microbial communities in a global challenge by analyzing the transcription factors network in a metagenome.