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Use of agroindustrial lignocellulosic waste for the production of oligosaccharides aiming at obtaining a prebiotic agent in human health

Grant number: 17/24503-2
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2019
Effective date (End): February 28, 2021
Field of knowledge:Agronomical Sciences - Food Science and Technology
Principal Investigator:Rosana Goldbeck
Grantee:Patricia Felix Avila
Home Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:15/50612-8 - An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks, AP.TEM

Abstract

Lignocellulosic materials represent a source of under-exploited raw material in biotechnological processes, with the largest residue surplus being the industrialization of basic products. In this way, the production of Xyloligosaccharides (XOs) from the enzymatic hydrolysis of xylan presents a hemicellulose from its residues, as well as the Cello-Oligosaccharides (CELOs), as a part of the cellulose presents a smart and sustainable alternative for their valorization, as well as in the production of industrial interest products with high added value. Xylo-Oligosaccharides (XOs) are distinguished by their prebiotic activities that favor an improvement in intestinal functions, an immunological, antimicrobial action and other health benefits. In this sense, the present proposal aims to produce oligosaccharides (XOs and CELOs) with prebiotic potential from the enzymatic hydrolysis of sugarcane straw. In the first stage, a DCCR (composite, central and rotational) type of experimental design will be carried out in order to select and optimize the optimum concentrations of the enzymes in the release of XOs and CELOs with polymerization degree (GP) of 2 to 6. In the first stage, a DCCR (composite, central and rotational) type of experimental design will be carried out in order to select and optimize the optimal concentrations of the enzymes in the release of XOs and CELOs with polymerization degree (GP) of 2 to 6. Optimized the production of oligosaccharides, these will be purified using a tangential system with nanofiltration membranes aiming at the separation of monosaccharides and inhibitory compounds present. After the purification step, the prebiotic potential of the oligosaccharides obtained by in vitro digestibility tests and in vitro fermentation with probiotic bacteria of the genus Lactobacillus and Bifidobacterium will be investigated. Finally, in vivo tests will be performed through the insertion of the oligosaccharides produced in a previously standardized diet in growing rats. Through the biological assays we intend to determine the anti-oxidant potential of the oligosaccharides produced in the tissues of organs (intestine, pancreas liver and spleen), as well as the growth of probiotic bacteria of the genus Lactobacillus and Bifidobacterium in the cecal lumial material. In parallel, the short chain fatty acids produced during growth will be quantified so that we can in fact prove the prebiotic power of the XOs and CELOs obtained. It is important to point out that this project has an innovative character, as it aims not only to produce high value-added bioproducts from lignocellulosic agroindustrial waste, but also aims to evaluate the prebiotic potential of the oligos produced for human health, through in vitro "and" in vivo. In addition, little is discussed in the literature regarding the prebiotic potential of cello-oligosaccharides. Thus, it is expected at the end of the project that the oligos produced from sugarcane straw present a great contribution in human health so that they may indeed have industrial application as functional ingredients in food products. We would also like to mention that this project is directly integrated into the FAPESP-BBSRC Thematic Project (2015/50612-8) entitled "An integrated approach to explore a novel paradigm for biofuel production from lignocellulosic feedstocks", in which our objectives (production and purification of oligosaccharides from lignocellulosic agroindustrial waste) will greatly contribute to the development of the aforementioned thematic project, in which we are inserted. (AU)