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Tribbles 3 role in skeletal muscle regeneration

Grant number: 18/24418-8
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): January 01, 2019
Effective date (End): September 30, 2021
Field of knowledge:Biological Sciences - Morphology - Anatomy
Principal researcher:Anselmo Sigari Moriscot
Grantee:Wenddy Wyllie Damascena Sougey
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:15/04090-0 - Identification and caracterization of mechanisms involved in skeletal muscle mass control and regeneration, AP.TEM

Abstract

The skeletal muscle tissue has, in addition to locomotor function, endocrine functions and energy storage, having great importance for daily life activity and quality of life. For being intensely recruited, it is quite susceptible to lesions that can compromise the tissue components and, in the long run, cause functional deficit to the body. For this not to happen, this tissue has regenerative plastic capacity, mainly through myogenic cells, called satellites. After a lesion, the skeletal muscle goes through the inflammatory, proliferative and remodeling phases of the fibers, giving this tissue a great capacity for regeneration. Previous studies in our group (unpublished data) have shown increased expression of the TRIB3 gene during the muscle atrophic process. Its participation in the skeletal muscle metabolism is still very little elucidated and is related to the decrease in the formation of myotubes. In this way, this work aims to investigate the role of TRIB3 on the regenerative process. More specifically, to characterize the location of TRIB3 in healthy muscle fiber and during regeneration, in addition to its predominance in different types of muscle fibers, to analyze the participation of TRIB3 in C2C12 cell differentiation, muscle regeneration after cardiotoxin injury in TRIB3-knockout mice, Nude mice and Mice hyperexpressing TRIB3. We also aim to investigate the regulation of the gene in question through the analysis and validation of potentially regulatory microRNAs. (AU)