Hybrid hydrogels for local and prolonged nitric oxide release

• Grant number: 18/14142-5
• Support Opportunities: Scholarships in Brazil - Post-Doctorate
• Effective date (Start): February 01, 2019
• Effective date (End): January 31, 2023
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Marcelo Ganzarolli de Oliveira
• Grantee: Laura Caetano Escobar da Silva
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 16/02414-5 - Topical and absorbable biomaterials for the local nitric oxide release, AP.TEM

Abstract

The aim of this project is to produce and characterize self-organized and multicomponent hybrid hydrogels that are able to locally deliver nitric oxide (NO) in a prolonged fashion. NO is one of the smallest known biologically active molecules which participates in a series of physiological and pathophysiological processes, such as epithelial tissue regeneration, vasodilation and, in increased concentrations, may lead to cell death by apoptosis. The NO ability to promote apoptosis has recently been proven to be useful for the development of antitumoral devices. In this context, the ability to control NO concentration, as well as NO release rate from biomaterials is essential. Moreover, NO low residence time in corporeal media demands for biomaterials that allow the NO release to be prolonged for days or even weeks. Therefore, the present project proposes the development of hybrid hydrogels cross-linked by silsesquioxane structures, that incorporate distinct NO donors (hydrophilic and hydrophobic) to promote a two-step NO release as well as to prolong NO release. The hybrid hydrogels of interest to this work will be prepared by means of the hydrolysis and condensation of triethoxysilyl groups, chemically linked to the polymeric chains and will generate hydrogels for topical applications and nanogels for antitumoral applications. Finally, the materials obtained herein will be characterized in terms of their physical-chemical properties and NO release profiles both n vitro and in vivo.

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PICHETH, GUILHERME F.; DA SILVA, LAURA C. E.; GIGLIO, LEONARDO P.; PLIVELIC, TOMAS S.; DE OLIVEIRA, MARCELO G.. S-nitrosothiol-terminated Pluronic F127: Influence of microstructure on nitric oxide release. Journal of Colloid and Interface Science, v. 576, p. 457-467, SEP 15 2020. (18/14142-5, 17/04622-7, 16/02414-5, 17/19253-7)

POVOA, VALERIA C. O.; DOS SANTOS, GIOVANNA J. V. P.; PICHETH, GUILHERME F.; JARA, CARLOS P.; DA SILVA, LAURA C. E.; DE ARAUJO, ELIANA P.; DE OLIVEIRA, MARCELO G.. Wound healing action of nitric oxide-releasing self-expandable collagen sponge. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v. 14, n. 6, MAY 2020. (13/07607-8, 18/14142-5, 17/04615-0, 16/02414-5, 17/19253-7)

GARZON-PORRAS, ANA M.; BERTUZZI, DIEGO L.; LUCAS, KURT; DA SILVA, LAURA C. E.; DE OLIVEIRA, MARCELO G.; ORNELAS, CATIA. Nitric Oxide Releasing Polyamide Dendrimer with Anti-inflammatory Activity. ACS APPLIED POLYMER MATERIALS, v. 2, n. 5, p. 2027-2034, MAY 2020. (18/14142-5, 18/02093-0, 15/23761-2, 15/04929-0, 16/02414-5)

SANTOS, MURILO I.; DA SILVA, LAURA C. E.; BOMEDIANO, MATEUS P.; CATORI, DANIELE M.; GONCALVES, MARIA C.; DE OLIVEIRA, MARCELO G.. 3D printed nitric oxide-releasing poly(acrylic acid)/F127/cellulose nanocrystal hydrogels. SOFT MATTER, v. 17, n. 26, p. 6352-6361, JUL 14 2021. (18/14142-5, 19/07325-9, 16/02414-5)

DA SILVA, LAURA C. E.; BORGES, ANTONIO C.; DE OLIVEIRA, MARCELO G.; DE FARIAS, MARCELO A.. Visualization of supramolecular structure of Pluronic F127 micellar hydrogels using cryo-TEM. METHODSX, v. 7, 2020. (16/02414-5, 18/14142-5)