Sources, Targets and Biological Responses of Triplet Species and Singlet Oxygen: Pathogenicity and Quenching.

• Grant number: 18/25842-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: February 01, 2019
• End date: October 31, 2019
• Field of knowledge: Biological Sciences - Biophysics - Radiology and Photobiology
• Principal Investigator: Etelvino José Henriques Bechara
• Grantee: Leticia Christina Pires Gonçalves
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 17/22501-2 - Electronic Chemiexcitation in Biological Systems: Bioluminescence and Photochemistry in The Dark, AP.TEM

Abstract

The Fungal Bioluminescence Laboratory (FBL) of the Chemistry Institute/University of São Paulo (IQ-USP), through the Thematic project of FAPESP "Electronic Chemiexcitation in Biological Systems: Bioluminescence and 'Photochemistry in the Dark'", offers two Post-doctoral positions for candidates with a PhD degree obtained for a maximum of 7 (seven) years. It has been proposed to investigate mechanisms of radical or enzymatic generation of electron-chemiexcited species in biological systems, involved in damage of proteins and DNA thereby triggering pathogenic responses. Among them, we aim to study (i) the mutagenic cyclobutane dimerization of DNA pyrimidines in the absence of light and their quenching by sorbates, (ii) modification of hemeproteins (HRP, myoglobin, hemoglobin) in the presence of H2O2 with loss of peroxidase activity, (iv) search of new sources of singlet oxygen, specifically acrolein present in thermoprocessed food and formylhydrazine found in some poisoning mushrooms (e.g., Gyromitra esculenta), and (iv) enzymatic generation of triplet carbonyl compounds in model systems for oxidative and carbonyl stress. Kinetic studies, isotopic labelling and conventional spectroscopic, photochemical and photophysical methods are experimental approaches in the development of the project. Our project aims to accumulate knowledge underlying the chemiexcitation field in biomedicine, develop bioanalytical tools, and foster international collaborations.

Scientific publications

(4)

(The scientific publications listed on this page originate from the Web of Science or SciELO databases. Their authors have cited FAPESP grant or fellowship project numbers awarded to Principal Investigators or Fellowship Recipients, whether or not they are among the authors. This information is collected automatically and retrieved directly from those bibliometric databases.)

GONCALVES, LETICIA C. P.; MASSARI, JULIO; LICCIARDI, SAYMON; PRADO, FERNANDA M.; LINARES, EDLAINE; KLASSEN, ALINE; TAVARES, MARINA F. M.; AUGUSTO, OHARA; DI MASCIO, PAOLO; BECHARA, ETELVINO J. H.. Singlet oxygen generation by the reaction of acrolein with peroxynitrite via a 2-hydroxyvinyl radical intermediate. Free Radical Biology and Medicine, v. 152, p. 83-90, MAY 20 2020. (18/25842-8, 12/12663-1, 13/07937-8, 17/27059-6, 17/22501-2)

FREITAS-DORR, B. C.; MACHADO, C. O.; PINHEIRO, A. C.; FERNANDES, A. B.; DORR, F. A.; PINTO, E.; LOPES-FERREIRA, M.; ABDELLAH, M.; SA, J.; RUSSO, L. C.; et al. A metal-free blue chromophore derived from plant pigments. SCIENCE ADVANCES, v. 6, n. 14, APR 2020. (18/25842-8, 14/10563-5, 16/21445-9, 15/25629-4, 14/22136-4, 19/06391-8, 15/24760-0, 15/18474-4)

SOARES, DOUGLAS M. M.; GONCALVES, LETICIA C. P.; MACHADO, CAROLINE O.; ESTEVES, LARISSA C.; STEVANI, CASSIUS, V; OLIVEIRA, CARLA C.; DORR, FELIPE A.; PINTO, ERNANI; ADACHI, FLAVIA M. M.; HOTTA, CARLOS T.; et al. Reannotation of Fly Amanita L-DOPA Dioxygenase Gene Enables Its Cloning and Heterologous Expression. ACS OMEGA, v. 7, n. 18, p. 10-pg., 2022-05-10. (19/12605-0, 19/15412-9, 17/22501-2, 18/25842-8, 15/24760-0, 13/07914-8, 19/06391-8)

GONCALVES, LETICIA C. P.; ANGELE-MARTINEZ, CARLOS; PREMI, SANJAY; PALMATIER, MEG A.; PRADO, FERNANDA MANSO; DI MASCIO, PAOLO; BASTOS, ERICK L.; BRASH, DOUGLAS E.. Chemiexcited Neurotransmitters and Hormones Create DNA Photoproducts in the Dark. ACS Chemical Biology, v. N/A, p. 10-pg., 2023-02-12. (18/25842-8, 19/15412-9, 12/12663-1)