Evaluation of autophagic flow in response to cellular stress of musculoskeletal and adipose tissues in obese patients with obstructive sleep apnea

• Grant number: 18/15921-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: January 01, 2019
• End date: March 31, 2022
• Field of knowledge: Health Sciences - Medicine
• Principal Investigator: Lia Rita Azeredo Bittencourt
• Grantee: Marcos Mônico Neto
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

Introduction: Obstructive sleep apnea (OSA) is a sleep-disordered breathing whose main risk factor isthe obesity. Changes in metabolic and inflammatory profile induced by OSA and obesity has beenpointed as responsible by increase of comorbidities, such as insulin resistance and type 2 diabetesmellitus. Thus, the skeletal muscle and adipose tissues are key components in metabolic regulation andinsulin sensibility, producing and releasing factors that act of autocrine, paracrine and endocrine mannerto adaptation of energy system in cellular stress moment. The autophagy contributes with this processand can be altered in obese subjects and potentiated in apneic patients, since OSA potentiate the proinflammatoryprofile induced by obesity, exposes the subjects to intermittent hypoxia during sleep andincrease the sympathetic tone activity, generating high rest metabolic rate.Aim: to evaluate metabolic regulation factors and autophagy from visceral adipose and skeletal muscleof obese individuals with OSA, in a stress cellular condition induced by fasting.Methods: Obese individuals who will undergo to bariatric and metabolic surgery (BMIe40 or e35 withassociated comorbidities). The volunteers will be distributed in six groups, non-apneic, mild apneic,moderate apneic and severe apneic. There will also be a non-apneic and apneic eutrophic control groupscomposed of patients who will be referred for cholecystectomy. The subjects will be evaluate toinflammatory profile, body mass, rest metabolic rate, autophagic flux from skeletal muscle and adiposesample collected in fast condition, during intraoperative moment.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MONICO-NETO, MARCOS; LEE, KIL SUN; DA LUZ, MARCIO HENRIQUE MELLO; PINO, JESSICA MONTEIRO VOLEJNIK; RIBEIRO, DANIEL ARAKI; CARDOSO, CAROLINE MARGONATO; LE SUEUR-MALUF, LUCIANA; TUFIK, SERGIO; ANTUNES, HANNA KAREN MOREIRA. Histopathological changes and oxidative damage in type I and type II muscle fibers in rats undergoing paradoxical sleep deprivation. CELLULAR SIGNALLING, v. 81, MAY 2021. (13/00152-5, 18/15921-8)

FLYGARE SOUZA, ANA CAROLINA; MONICO-NETO, MARCOS; LE SUEUR-MALUF, LUCIANA; MAZZUCO PIDONE, FLAVIA ANDRESSA; MOREIRA ANTUNES, HANNA KAREN; RIBEIRO, DANIEL ARAKI. Inflammatory activity and apoptosis are associated with tissue degeneration in the submandibular gland of rats submitted to paradoxical sleep deprivation. ODONTOLOGY, v. 110, n. 2, p. 9-pg., 2022-04-01. (18/15921-8)

PINO, JESSICA M. V.; SILVA, VITORIA F.; MONICO-NETO, MARCOS; SEVA, DANIELLE C.; KATO, MELISSA Y.; ALVES, JULY N.; PEREIRA, GABRIELA C.; ANTUNES, HANNA KAREN M.; GALVAO, THALES D.; BITTERNCOURT, LIA R. A.; et al. Severe Obesity in Women Can Lead to Worse Memory Function and Iron Dyshomeostasis Compared to Lower Grade Obesity. INTERNATIONAL JOURNAL OF ENDOCRINOLOGY, v. 2023, p. 12-pg., 2023-04-17. (19/21511-0, 18/15921-8)

SEVA, DANIELLE CRISTINA; MONICO-NETO, MARCOS; ANTUNES, HANNA KAREN MOREIRA; PINO, JESSICA MONTEIRO VOLEJNIK; BITTENCOURT, LIA RITA AZEREDO; GALVAO, THALES DELMONDES; DAMASO, ANA R.; OYAMA, LILA MISSAE; SHIVAPPA, NITIN; HEBERT, JAMES R.; et al. Beneficial Short-Term Effects of Bariatric Surgery on Nutritional Inflammatory Profile and Metabolic Biomarkers. OBESITY SURGERY, v. 33, n. 9, p. 10-pg., 2023-08-04. (18/15921-8)