Acute toxicity evaluation of Stizophyllum perforatum leaves crude ethanol extract in vivo and chemical study associated with the evaluation of antiparasitic and cytotoxic activities in vitro

• Grant number: 17/24860-0
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• Effective date (Start): February 01, 2019
• Effective date (End): January 31, 2024
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Patricia Mendonça Pauletti
• Grantee: Osvaine Junior Alvarenga Alves
• Host Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

The chemical study and the evaluation of the biological potential of Stizophyllum perforatum leaves ethanol crude extract were carried out by our research group, since S. perforatum presented leishmanicidal activity, with a selectivity index of 28.67, when evaluated in a normal cell line. Therefore, the extract was considered promising and selective for the parasite. The EtOAc active fraction, against this protozoan, was purified and resulted, until now, in the isolation of ursolic acid, oleanolic acid and verbascoside, other compounds were also isolated, but are under chemical structural determination. This research project aims to continue studies with S. perforatum in order to evaluating the acute toxicity of the active extract, obtain a fingerprint of the extract by HPLC-DAD and HPLC-MS and quantification of, the chemical marker, verboscoside, isolating and identifying the bioactive compounds from S. perforatum ethanolic extract with potential use as leishmanicide and antimalarial, as well as to evaluate the cytotoxic properties. The analytical methodologies to be used in the phytochemical study will be chromatographic techniques (LC, TLC, GC-MS, HPLC-DAD, and HPLC-MS), and compounds will be identified by spectroscopic methods. Lastly, this project will also contribute to the research project "Phytochemistry study and evaluation of the schistosomicidal, cytotoxicity and leishmanicidal activities from Cerrado plants." (Grant number: 2016/10313-4), to the human resource formation, and with the UNIFRAN postgraduate program in Science (Capes, score 5). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ALVARENGA, TAVANE APARECIDA; ALVARENGA ALVES, OSVAINE JUNIOR; PAGOTTI, MARIANA CINTRA; CUNHA, WILSON ROBERTO; ANDRADE E SILVA, MARCIO LUIS; SALES, JULIANA DE FATIMA; SILVA, FABIANO GUIMARAES; JANUARIO, ANA HELENA; MAGALHAES, LIZANDRA GUIDI; PAULETTIA, PATRICIA MENDONCA. In vitro antileishmanial activity of Anacardium othonianum and isolated compounds against Leishmania amazonensis. ACTA BRASILIENSIS, v. 5, n. 1, p. 44-47, JAN-APR 2021. (16/10313-4, 17/24860-0)

JOÃO PAULO CAMPOS ANDRADE; OSVAINE JUNIOR ALVARENGA ALVES; MARIAN CAROLINE COSTA; VALÉRIA MARIA MELLEIRO GIMENEZ; IARA SILVA SQUARISI; HELOIZA DINIZ NICOLELLA; MARIANA CINTRA PAGOTTI; DENISE CRISPIM TAVARES; WILSON ROBERTO CUNHA; MÁRCIO LUÍS ANDRADE E SILVA; et al. Chemical study of Adenocalymma axillarum crude leaf extract and isolated compounds. Brazilian Journal of Pharmaceutical Sciences, v. 58, 2020-11-14. (16/10313-4, 17/24860-0)

MILANI, REINALDO R.; ALVES, OSVAINE J. A.; BERTANHA, CAMILA S.; GIMENEZ, VALERIA M. M.; SQUARISI, IARA S.; NICOLELLA, HELOIZA D.; PAGOTTI, MARIANA C.; TAVARES, DENISE C.; E SILVA, MARCIO L. A.; CUNHA, WILSON R.; et al. ANTIPROMASTIGOTE AND CYTOTOXIC ACTIVITIES OF FLAVONOIDS FROM Fridericia speciosa LEAVES. Química Nova, v. 43, n. 10, p. 1404-1407, DEC 2020. (15/04999-8, 16/10313-4, 13/09280-6, 17/24860-0)