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Acute toxicity evaluation of Stizophyllum perforatum leaves crude ethanol extract in vivo and chemical study associated with the evaluation of antiparasitic and cytotoxic activities in vitro

Grant number: 17/24860-0
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): February 01, 2019
Effective date (End): January 31, 2023
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Organic Chemistry
Principal Investigator:Patricia Mendonça Pauletti
Grantee:Osvaine Junior Alvarenga Alves
Home Institution: Pró-Reitoria Adjunta de Pesquisa e Pós-Graduação. Universidade de Franca (UNIFRAN). Franca , SP, Brazil

Abstract

The chemical study and the evaluation of the biological potential of Stizophyllum perforatum leaves ethanol crude extract were carried out by our research group, since S. perforatum presented leishmanicidal activity, with a selectivity index of 28.67, when evaluated in a normal cell line. Therefore, the extract was considered promising and selective for the parasite. The EtOAc active fraction, against this protozoan, was purified and resulted, until now, in the isolation of ursolic acid, oleanolic acid and verbascoside, other compounds were also isolated, but are under chemical structural determination. This research project aims to continue studies with S. perforatum in order to evaluating the acute toxicity of the active extract, obtain a fingerprint of the extract by HPLC-DAD and HPLC-MS and quantification of, the chemical marker, verboscoside, isolating and identifying the bioactive compounds from S. perforatum ethanolic extract with potential use as leishmanicide and antimalarial, as well as to evaluate the cytotoxic properties. The analytical methodologies to be used in the phytochemical study will be chromatographic techniques (LC, TLC, GC-MS, HPLC-DAD, and HPLC-MS), and compounds will be identified by spectroscopic methods. Lastly, this project will also contribute to the research project "Phytochemistry study and evaluation of the schistosomicidal, cytotoxicity and leishmanicidal activities from Cerrado plants." (Grant number: 2016/10313-4), to the human resource formation, and with the UNIFRAN postgraduate program in Science (Capes, score 5). (AU)