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Whole genome sequencing and comparative genomics of Campylobacter coli strains isolated from different sources over a 16 year-period in Brazil

Grant number: 18/26043-1
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): May 01, 2019
Effective date (End): October 31, 2019
Field of knowledge:Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
Principal Investigator:Juliana Pfrimer Falcão
Grantee:Carolina Nogueira Gomes
Supervisor abroad: Eduardo Napoleon Taboada
Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Local de pesquisa : Public Health Agency of Canada Manitoba, Canada  
Associated to the scholarship:15/23408-0 - Phenotypic analysis, genome and transcriptome sequencing of Campylobacter coli strains isolated from different sources during 16 years in Brazil, BP.DR

Abstract

Campylobacteriosis is a zoonosis of worldwide distribution with significant repercussions at the level of Public Health and with a high socio-economic impact. Considering that Brazil is the largest exporter of poultry meat in the world, and that the most important potential transmission routes of Campylobacter coli to humans are considered to be the consumption and handling of improperly prepared poultry meat, campylobacteriosis has been a neglected disease and there is insufficient data to estimate the incidence of this pathogen in this country. Furthermore, the research conducted in Brazil has mostly focused on the occurrence and antimicrobial resistance of C. coli and C. jejuni and there are few studies in which Campylobacter spp. strains have been subjected to molecular characterization. Thus, molecular studies that have the purpose to elucidate the pathogenesis and characterize Campylobacter coli strains are of great importance, especially in Brazil where there is a paucity of data for this pathogen of worldwide importance. This project aims to perform whole-genome sequencing and comparative genomic analysis of C. coli strains isolated over a 16-year period in Brazil. For this, we will study 63 C. coli strains isolated from humans (12), animals (21), the environment (20) and food (10) from some cities in Brazil between 1995-2011. All the 63 C. coli strains will have their whole genome sequenced using the NextSeq - Illumina platform during the training in the Public Health Agency of Canada; National Microbiology Laboratory under supervision of Dr. Eduardo N. Taboada. The comparative analysis of the whole genome sequences of the C. coli strains to be studied will provide important data on genetic variation, including: differences in gene content; and differences in insertions, deletions and/or point mutations that might affect the expression and/or regulation of specific genes. Moreover, these analyses may help to elucidate possible mechanisms that influence public health outcomes through effects on pathogenicity/virulence and survival along the poultry chain in C. coli strains. Together, the results obtained will contribute to a better understanding of C. coli, as well as will provide information about the strains circulating in our country.