Advanced search
Start date
Betweenand

Investigation of small molecules signaling networks on citrus sinensis infected with Xanthomonas citri sub. citri using NMR and UHPLC-TOF/MS based metabolomics

Grant number: 19/00307-5
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): May 01, 2019
Effective date (End): November 30, 2019
Field of knowledge:Agronomical Sciences - Agronomy
Principal Investigator:Henrique Ferreira
Grantee:Luiz Leonardo Saldanha
Supervisor abroad: Jean-Luc Wolfender
Home Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
Local de pesquisa : Université de Genève, Switzerland  
Associated to the scholarship:18/10734-5 - Metabolic profiles of Xanthomonas citri sub. citri, and the plant-host response upon infection with the phytopathogen, BP.PD

Abstract

Citrus canker, caused by the bacteria Xanthomonas citri subsp. citri, is one of the most aggressive plant diseases and affects all Citrus commodities worldwide. This disease has a great impact on production and quality of the fruit, preventing the trade and causing great losses to agriculture in Brazil, the world's largest sweet orange producer. In São Paulo state, the easing of citrus canker control laws and the lack of knowledge about functional aspects of this disease, culminate in a significant incidence and endemic status in this state. Currently application of copper sprays in crops is the unique preventive control of citrus canker, other than plant eradication, and may led to accumulation of this metal in environment and copper tolerance and resistance strains. Research for citrus crops protection, recommend the development of bio based processes and technologies environmentally friendly rather than the use of agrochemicals. Therefore, the elucidation of functional aspects of Citrus-Xanthomonas during disease development will generate basics concepts of plant defense mechanism to development of new citrus verities less susceptible to citrus canker in long-term basis. Molecular aspects of this disease have greatly expanded recently, however, small molecules used by plants to encode signals acquired by pathogen recognition to activation of defense response genes and protection have been poorly explored. Through the study of metabolomics using LC-HRMS and Nuclear magnetic resonance (NMR) followed by multivariate statistical analysis, we expect to characterize small molecules networking involved in different mechanisms of C. sinenis responses to X. citri infection generating novel and in depth information of poorly studied aspects of citrus canker disease.