Investigation of small molecules signaling networks on Citrus sinensis infected with Xanthomonas citri sub. citri using NMR and UHPLC-TOF/MS based metabolomics

• Grant number: 19/00307-5
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: May 01, 2019
• End date: November 30, 2019
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Henrique Ferreira
• Grantee: Luiz Leonardo Saldanha
• Supervisor: Jean-Luc Wolfender
• Host Institution: Instituto de Biociências (IB). Universidade Estadual Paulista (UNESP). Campus de Rio Claro. Rio Claro , SP, Brazil
• Institution abroad: Université de Genève, Switzerland
• Associated to the scholarship: 18/10734-5 - Metabolic profiles of Xanthomonas citri sub. citri, and the plant-host response upon infection with the phytopathogen, BP.PD

Abstract

Citrus canker, caused by the bacteria Xanthomonas citri subsp. citri, is one of the most aggressive plant diseases and affects all Citrus commodities worldwide. This disease has a great impact on production and quality of the fruit, preventing the trade and causing great losses to agriculture in Brazil, the world's largest sweet orange producer. In São Paulo state, the easing of citrus canker control laws and the lack of knowledge about functional aspects of this disease, culminate in a significant incidence and endemic status in this state. Currently application of copper sprays in crops is the unique preventive control of citrus canker, other than plant eradication, and may led to accumulation of this metal in environment and copper tolerance and resistance strains. Research for citrus crops protection, recommend the development of bio based processes and technologies environmentally friendly rather than the use of agrochemicals. Therefore, the elucidation of functional aspects of Citrus-Xanthomonas during disease development will generate basics concepts of plant defense mechanism to development of new citrus verities less susceptible to citrus canker in long-term basis. Molecular aspects of this disease have greatly expanded recently, however, small molecules used by plants to encode signals acquired by pathogen recognition to activation of defense response genes and protection have been poorly explored. Through the study of metabolomics using LC-HRMS and Nuclear magnetic resonance (NMR) followed by multivariate statistical analysis, we expect to characterize small molecules networking involved in different mechanisms of C. sinenis responses to X. citri infection generating novel and in depth information of poorly studied aspects of citrus canker disease.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SALDANHA, LUIZ LEONARDO; ALLARD, PIERRE-MARIE; DILARRI, GUILHERME; CODESIDO, SANTIAGO; GONZALEZ-RUIZ, VICTOR; QUEIROZ, EMERSON FERREIRA; FERREIRA, HENRIQUE; WOLFENDER, JEAN-LUC. Metabolomic- and Molecular Networking-Based Exploration of the Chemical Responses Induced in Citrus sinensis Leaves Inoculated with Xanthomonas citri. Journal of Agricultural and Food Chemistry, v. N/A, p. 13-pg., 2022-11-09. (14/50880-0, 19/00307-5, 15/50162-2)