he white spot lesion (LMB) can be defined as the primary clinical manifestation of dental caries, whose milky white appearance without dullness results from the loss of subsurface mineral structure. Based on this, the objective of this work will be to develop in vitro reproducible white spot lesions (LMB) with different levels of mineral loss using a microcosm biofilm model in human enamel samples and characterize them by microregion demineralization analysis (TMR), quantitative light-induced fluorescence (QLF) and spectrophotometry analysis (EasyShade), B) Compare lesions produced in vitro with natural LMBs in vivo using QLF and spectrophotometry (EasyShade). For phase A will be used 90 human enamel specimens where the microcosm biofilm model will be used and the demineralization analysis will be performed. For project B, we will select five volunteers who have just finished orthodontic treatment, where we will compare the lesions produced in vitro with natural LMBs analyzed using QLF and Easyshade. The data obtained will pass through the Kolmogorov and Smirnov and Bartlett tests to verify their normality and homogeneity, respectively. After this verification, the data will be submitted to statistical analysis (p <0.05).
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