Bioprospection of natural products in photodynamic therapy against microorganisms of medial and dental importance

• Grant number: 18/18440-0
• Support Opportunities: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2019
• Effective date (End): February 29, 2020
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Fernanda Lourenção Brighenti
• Grantee: Analú Barros de Oliveira
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

The interest for natural photosensitizers has increased in the past years due to its ease to adhere or pass through cytoplasmic membrane and due to its potential in production reactive oxygen species. The aim of this study is to evaluate the in vitro potential of essential oils and plant extracts to be used in photodynamic therapy as monotherapy on Streptococcus mutans, Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Candida albicans and Cutibacterium acnes. First, antimicrobial photodynamic therapy will be carried out using the microorganisms in suspension at sub-inhibitory concentration of the plant material. Four treatment groups will be studied: negative control, plant material, light and photodynamic therapy (light + plant material). The plant material with the best results will be tested using the microorganisms in biofilms. The efficacy of the therapy will be evaluated by viable cell counting after the treatment. The evaluation of the production of reactive oxygen species and the evaluation of cytotoxicity will be performed in plant materials that present the best antimicrobial activity.Data will be analyzed with GraphPad Prism versão 3.02 at 5% significance. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MIRANDA, MARINA LINS; SILVA, BIANCA NUBIA SOUZA; SALOMAO, KARINA BORGES; DE OLIVEIRA, ANALU BARROS; GABBAI-ARMELIN, PAULO ROBERTO; BRIGHENTI, FERNANDA LOURENCAO. Effect of arginine on microorganisms involved in dental caries: a systematic literature review ofin vitrostudies. BIOFOULING, AUG 2020. (13/12326-8, 18/18440-0)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; SILVEIRA, HEITOR ALBERGONI; SILVA, EVANIO VILELA; BUFALINO, ANDREIA; LEON, JORGE ESQUICHE; BRIGHENTI, FERNANDA LOURENCAO. Intraoral atypical lentiginous melanocytic lesion in a pediatric patient. Oral Oncology, v. 112, JAN 2021. (16/11419-0, 18/18440-0)

ANALÚ BARROS DE OLIVEIRA; TÚLIO MORANDIN FERRISSE; KARINA BORGES SALOMÃO; MARINA LINS MIRANDA; ANDREIA BUFALINO; FERNANDA LOURENÇÃO BRIGHENTI. Photobiomodulation in the treatment of xerostomia associated with hyposalivation in a pediatric patient with systemic scleroderma. Autopsy and Case Reports, v. 11, 2020-12-14. (18/18440-0)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; MARQUES, RAQUEL SOUZA; DE ANNUNZIO, SARAH RAQUEL; BRIGHENTI, FERNANDA LOURENCAO; FONTANA, CARLA RAQUEL. Effect of Photodynamic Therapy on Microorganisms Responsible for Dental Caries: A Systematic Review and Meta-Analysis. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 20, n. 14, JUL 2 2019. (18/23015-7, 18/18440-0, 18/09088-1)

GABBAI-ARMELIN, PAULO ROBERTO; DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; SALES, LUCIANA SOLERA; BARBOSA, ELIS RODRIGUES OLIVEIRA; MIRANDA, MARINA LINS; SALOMAO, KARINA BORGES; BRIGHENTI, FERNANDA LOURENCAO. COVID-19 (SARS-CoV-2) infection and thrombotic conditions: A systematic review and meta-analysis. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, v. 51, n. 6, APR 2021. (21/01191-0, 19/08375-0, 19/26066-4, 18/18440-0, 20/07110-0)

FERRISSE, TULIO MORANDIN; DE OLIVEIRA, ANALU BARROS; PALACON, MARIANA PARAVANI; SILVA, EVANIO VILELA; SGAVIOLI MASSUCATO, ELAINE MARIA; DE ALMEIDA, LUCIANA YAMAMOTO; LEON, JORGE ESQUICHE; BUFALINO, ANDREIA. The role of CD68+and CD163+macrophages in immunopathogenesis of oral lichen planus and oral lichenoid lesions. Immunobiology, v. 226, n. 3, MAY 2021. (20/07110-0, 16/11419-0, 17/01438-0, 18/18440-0)

DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; BASSO, FERNANDA GONCALVES; FONTANA, CARLA RAQUEL; APARECIDA GIRO, ELISA MARIA; BRIGHENTI, FERNANDA LOURENCAO. A systematic review and meta-analysis of the effect of photodynamic therapy for the treatment of oral mucositis. Photodiagnosis and Photodynamic Therapy, v. 34, JUN 2021. (18/18440-0, 20/07110-0, 21/01191-0, 19/08375-0, 18/23015-7)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

OLIVEIRA, Analú Barros de. Bioprospecting of natural products in photodynamic therapy against microrganisms of medical and dental interest. 2020. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.