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Study of the enriched-pathways in immune cells during early pregnancy and new pregnancy markers in the whole blood of bovine females

Grant number: 19/02995-6
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): March 20, 2019
Effective date (End): August 11, 2019
Field of knowledge:Agronomical Sciences - Veterinary Medicine
Principal Investigator:Guilherme Pugliesi
Grantee:Cecilia Constantino Rocha
Supervisor abroad: Niamh Mary Forde
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Local de pesquisa : University of Leeds, England  
Associated to the scholarship:17/13994-5 - Study of transcriptomic profile in immune cells of peripheral blood during early pregnancy in beef heifers, BP.MS

Abstract

The identification of pregnancy status earlier than the current methods performed around the 30th day after insemination allows a reduction in the interval of inseminations, which can result in a shorter conception period and greater profitability to the livestock production system. The central thesis of the project is that biomarkers in the blood stimulated by the concept are detectable in early pregnancy. This may allow the development of an early and innovative method of pregnancy diagnosis in cattle. Thus, the main objective is to discover new markers of pregnancy and study the pathways involved with this new markers in immune cells, to serve as a basis for the development of molecular methods of early diagnosis of pregnancy in cattle. For this, on Study 1, the genes found in the transcriptome analysis through the seqRNA (master's experiment [experiment 1]), will be submitted to enrichment analyzes, for evaluation of the pathways, biological process and molecular functions enriched on the mononuclear immune cells of the blood (PBMCs) on pregnant and non-pregnant animals. On Study 2, some of genes found on the seqRNA, validated through the qPCR, that are great markers of pregnancy on the PBMCs (experiment 1), will be evaluated on the total blood of Holstein cows. The new knowledge and innovative methods proposed here will underpin the development of technologies for dairy beef cows.