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Uncovering pathophysiological and molecular mechanisms involvedin tumorigenesis by platforms for next-generation sequencing (NGS)

Grant number: 19/00860-6
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): March 01, 2019
Effective date (End): April 30, 2023
Field of knowledge:Health Sciences - Medicine - Medical Clinics
Principal Investigator:Sonir Roberto Rauber Antonini
Grantee:Ana Carolina Bueno de Queiroz Arruda
Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:14/03989-6 - Uncovering pathophysiological and molecular mechanisms involved in tumorigenesis by platforms for next-generation sequencing (NGS), AP.TEM

Abstract

To elucidate the molecular pathogenesis of endocrine tumors, several studies have investigated the role of specific genes regulating the organ development, genes involved in tumorigenesis of other tissues, such as abnormalities in genes that lead to cycle progression and cell division (oncogenes) or genes that block the cell cycle (tumor suppressor genes), miRNAs, and large noncoding (lncRNAs). Significant advances have been observed in the molecular elucidation of various hereditary syndromes that occur with pituitary tumors [(McCune - Albright, Multiple endocrine neoplasia (MEN) type 1 and type 4, Carney's syndrome, and familial isolated pituitary adenoma (FIPA)]; with adrenal tumors (Li-Fraumeni syndrome, Beckwith-Wiedemann syndrome, Carney complex, McCune-Albright, MEN1); and with thyroid tumors (MEN2, Hereditary medullar thyroid carcinoma). All these advances occurred due approaches based on candidate genes and linkage studies, but the molecular pathogenesis of sporadic endocrine tumors has not been completely clarified. The new platforms of next generation sequencing (NGS) present major advantages for the analysis of 1) exoma (Target-seq analysis) 2) epigenomic (either by chromatin immunoprecipitation (ChIP) or promoter DNA methylation analysis), and 3) transcriptome. Therefore, this project aims to use NGS technology to better understand the process of central nervous system (glyomas) and endocrine tumorigenesis. Tumoral tissues will be obtained from patients followed in the Hospital das Clinicas, Faculty of Medicine of Ribeirão Preto, University of São Paulo (HCFMRP-USP). As controls, normal tissue samples will be obtained at CEMEL (Center of Forensic Medicine) from autopsies of patients with accidental or sudden death from cardiac causes. The NGS certainly will indicate new genes and/or new cell signaling pathways that may be keys of the enlightenment of endocrine tumorigenesis.

News published in Agência FAPESP Newsletter about the scholarship:
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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BUENO, ANA CAROLINA; STECCHINI, MONICA F.; MARRERO-GUTIERREZ, JUNIER; MORE, CANDY BELLIDO; LEAL, LETICIA FERRO; GOMES, DEBORA CRISTIANE; DE LIMA NETO, DANIEL FERREIRA; BRANDALISE, SILVIA REGINA; CARDINALLI, IZILDA APARECIDA; YUNES, JOSE ANDRES; et al. Vitamin D receptor hypermethylation as a biomarker for pediatric adrenocortical tumors. EUROPEAN JOURNAL OF ENDOCRINOLOGY, v. 186, n. 5, p. 13-pg., . (15/19663-5, 20/03835-0, 14/03989-6, 19/00860-6)
BUENO, ANA CAROLINA; MORE, CANDY BELLIDO; MARRERO-GUTIERREZ, JUNIER; SILVA, DANILLO C. DE ALMEIDA E.; LEAL, LETICIA FERRO; MONTALDI, ANA PAULA; RAMALHO, FERNANDO SILVA; NICOLIELLO VENCIO, RICARDO ZORZETTO; DE CASTRO, MARGARET; ANTONINI, SONIR ROBERTO R.. Vitamin D receptor activation is a feasible therapeutic target to impair adrenocortical tumorigenesis. Molecular and Cellular Endocrinology, v. 558, p. 14-pg., . (21/04368-9, 15/19663-5, 17/17737-7, 22/04883-3, 14/03989-6, 19/00860-6)
SOARES MOTA, JOSE ITALO; PATRICIO SILVA-JUNIOR, RUI MILTON; MARTINS, CLARISSA SILVA; BUENO, ANA CAROLINA; WILDEMBERG, LUIZ EDUARDO; DA SILVA ANTUNES, XIMENE LIMA; OKANOBO OZAKI, JORGE GUILHERME; COELI-LACCHINI, FERNANDA BORCHERS; GARCIA-PERAL, CARLOS; ROCHA OLIVEIRA, ANTONIO EDSON; et al. Telomere length and Wnt/beta-catenin pathway in adamantinomatous craniopharyngiomas. EUROPEAN JOURNAL OF ENDOCRINOLOGY, v. 187, n. 2, p. 12-pg., . (19/00849-2, 14/03989-6, 19/00860-6)
BUENO, ANA CAROLINA; DA SILVA, RUI M. P.; STECCHINI, MONICA F.; MARRERO-GUTIERREZ, JUNIER; DE ALMEIDA E SILVA, DANILLO C.; CARDINALLI, IZILDA; SCRIDELI, CARLOS ALBERTO; JUNQUEIRA, THAIS; MOLINA, CARLOS A. F.; RAMALHO, FERNANDO SILVA; et al. DNA methylation is a comprehensive marker for pediatric adrenocortical tumors. Endocrine-Related Cancer, v. 29, n. 11, p. 15-pg., . (20/03835-0, 19/00860-6, 21/04368-9, 19/00849-2, 14/03989-6)

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