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Potential of different isolates of Metarhizium sp in the alternative control (induced resistance) of Phakopsora pachyrhizi in soybean plants

Grant number: 18/18393-2
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2019
Effective date (End): June 30, 2020
Field of knowledge:Agronomical Sciences - Agronomy - Plant Health
Principal researcher:Sérgio Florentino Pascholati
Grantee:Sabrina Holz
Home Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Associated scholarship(s):19/19767-6 - Determine the active ingredient(s) of the anti-rust activity in the Metarhizium sp. culture filtrate and explore the potential of direct dsRNA application for managing soybean rust disease, BE.EP.MS


The soybean rust, caused by Phakopsora pachyrhizi , Is one of the main diseases that affect the soybean crop, causing several losses. Thus, the search of new efficient management tools is increasing, notably the alternative methods as"Induced resistance and biological control", which consist in the use of agents to control a target pathogen. The entomopathogenic fungus Metarhizium sp. is used in the biological control of pests and exhibits the potential to be used against plant pathogens. Thus, the objective of this work is to evaluate the effect of preparations (media filtrate and conidial suspension) of three Metarhizium isolates (M. anisopliae, M. robertsii and Metarhizium indeterminated species 1) on the control of P. pachyrhizi, evaluating their potential use as inducer of systemic resistance, which activates the mechanisms of plant defense. For this purpose, susceptible plants (maintained under greenhouse conditions) and detached leaves (kept under laboratory conditions) will be used. Further studies regarding biochemical assays will be carried out to understand the basis of the phenomena. In vitro assays will be carried out to evaluate the inhibitory effect of the preparations on urediniospore germination. To evaluate the in vivo assays, the severity of the disease will be measure by using a diagrammatic scale. Based upon the results to be obtained, this project aim to contribute to the knowledge and development of an alternative control measure for the soybean rust. (AU)