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Antigenic characterization of the membrane proteins of Mycoplasma agalactiae and them role in virulence and pathogenicity in vitro

Grant number: 19/03425-9
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): May 22, 2019
Effective date (End): March 19, 2020
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal Investigator:Jorge Timenetsky
Grantee:Maysa Santos Barbosa
Supervisor abroad: Rohini Chopra-Dewasthaly
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Local de pesquisa : University of Veterinary Medicine, Vienna, Austria  
Associated to the scholarship:16/23306-6 - Functional characterization and evaluation of the antigenicity and immunogenicity of recombinant proteins of Mycoplasma agalactiae, BP.DR

Abstract

Contagious agalactia (CA), caused mainly by Mycoplasma agalactiae (Mag), is a severe disease of sheep and goats that takes a significant toll on livestock with high morbidity and mortality rates. CA, an OIE (World Organisation for Animal Health)-listed disease, is distributed worldwide and its occurrence is likely underestimated in Brazil. In the coming years, as the country moves towards excellence in livestock health, it will become imperative to detect and control CA in small ruminants. LPPs (lipoproteins) are important antigens of mollicutes and are anchored in the cytoplasmic membrane interacting directly with the host cell modulating the immune response and influencing the immunopathogenesis of the disease. In a preliminary study in our laboratory (FAPESP 2014/09425-7 and CNPq 443400/ 2014-8), coding DNA sequences (CDS) of the M. agalactiae reference strain PG2 (GenBank accession number: NC_009497.1) were analyzed by bioinformatics tools to evaluate their cellular location (extracellular, transmembrane, cytoplasmic), predict secondary structures, and to evaluate predictions of linear and conformational epitopes for B cells. Lastly, MAG_0795, MAG_3125 and P40 proteins were expressed and purified from a heterologous E. coli system and characterized as possible antigens after immunoassays (manuscript under preparation). Therefore, the objective of this study is to characterize the role of three selected recombinant membrane proteins of M. agalactiae (MAG_0795, MAG_3125 and P40), through modulation of the immune and apoptotic profile. In this way, knowing the lipoproteins and its role in triggering infection has relevance for future studies on diagnosis, prevention and eradication of diseases caused by M. agalactiae.