Non-ulcerated or atipycal Cutaneous Leishmaniasis caused by Leishmania (L.) infantum chagasi in the municipality of Amapala, Valle, Honduras: immunohistopathological characterization of skin lesions with emphasis in the participation of antigen presenting cells

• Grant number: 18/04698-6
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): March 01, 2019
• Effective date (End): November 30, 2021
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Marcia Dalastra Laurenti
• Grantee: Carmen Maria Sandoval Pacheco
• Host Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

In the Americas, especially in South America, infection with Leishmania (Leishmania) infantum chagasi causes subclinical manifestations and visceral leishmaniasis (LV), which when untreated is potentially fatal. In Honduras, infection by Leishmania (L.) infantum chagasi causes Visceral Leishmaniasis (VL), but non-ulcerated or atypical cutaneous leishmaniasis (LCNU) caused by this parasite species has also been reported. Cases of non-ulcerated or atypical cutaneous leishmaniasis (NUCL) have been reported, also in other countries of the region, such as Costa Rica, El Salvador and Nicaragua. In Honduras, there are well-identified endemic areas of this non-ulcerated clinical variant, located in the departments of Choluteca and Valle, as well as southern parts of the departments of Francisco Morazán, La Paz, El Paraíso and Intibucá. In this study, 20 skin biopsies will be used in the Laboratory of Pathology of Infectious Diseases of FMUSP, which have already been collected for the development of another project. After clinical and laboratory examination to confirm Leishmania infection, biopsies of skin lesions of these patients, which are defined as a painless, non-ulcerative papule, infiltrative plaque or nodule, erythematous or skin color, with or without the presence of halo hypo-pigmented, were collected, fixed in 10% buffered formol solution 0.01M and processed by the usual techniques of optical microscopy. Histological sections of the cutaneous lesions will be processed by the immunohistochemical reaction using the primary antibodies to Langerhans cell markers (CD1a, CD207) and dermal dendritic cells (CD11c, CD123, factor XIIIa); M1 macrophages (CD68) and M2 macrophages (CD163); and double-stain immunohistochemistry using iNOS markers and production of cytokines such as IL-12, TNF-±, IL-4, IL-10, TGF-² and IL-13. (AU)

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SANDOVAL PACHECO, CARMEN M.; ARAUJO FLORES, V, GABRIELA; GONZALEZ, KADIR; DE CASTRO GOMES, CLAUDIA M.; PASSERO, LUIZ F. D.; TOMOKANE, THAISE Y.; SOSA-OCHOA, WILFREDO; ZUNIGA, CONCEPCION; CALZADA, JOSE; SALDANA, AZAEL; et al. Macrophage Polarization in the Skin Lesion Caused by Neotropical Species of Leishmania sp. JOURNAL OF IMMUNOLOGY RESEARCH, v. 2021, APR 12 2021. (14/50315-0, 17/24834-9, 15/01154-7, 18/04698-6)

SANDOVAL, CARMEN; ARAUJO, GABRIELA; SOSA, WILFREDO; AVALOS, SARA; SILVEIRA, FERNANDO; CORBETT, CARLOS; ZUNIGA, CONCEPCION; LAURENTI, MARCIA. In situ cellular immune response in non-ulcerated skin lesions due to Leishmania (L.) infantum chagasi infection. Journal of Venomous Animals and Toxins including Tropical Diseases, v. 27, FEB 26 2021. (18/04698-6, 14/50315-0, 17/24834-9, 15/01154-7)

LAURENTI, MARCIA DALASTRA; SOSA-OCHOA, WILFREDO; ARAUJO FLORES, GABRIELA VENICIA; SANDOVAL PACHECO, CARMEN MARIA; TOMOKANE, THAISE YUMIE; DA SILVA OLIVEIRA, LUANDA MARA; ZUNIGA, CONCEPCION; SILVEIRA, FERNANDO TOBIAS; PEREIRA CORBETT, CARLOS EDUARDO. valuation of systemic immunity in atypical cutaneous leishmaniasis caused by Leishmania (L.) infantum chagas. PARASITE IMMUNOLOGY, v. 44, n. 1-2, NOV 2021. (17/24834-9, 14/50315-0, 18/04698-6)

CAMILA A CARDOSO; GABRIELA V ARAUJO; CARMEN M SANDOVAL; PAULA M NOGUEIRA; CONCEPCION ZÚNIGA; WILFREDO H SOSA-OCHOA; MÁRCIA D LAURENTI; RODRIGO P SOARES. Lipophosphoglycans from dermotropic Leishmania infantum are more pro-inflammatory than those from viscerotropic strains. Memórias do Instituto Oswaldo Cruz, v. 115, 2020-09-21. (14/50315-0, 18/04698-6, 17/24834-9)

PACHECO, CARMEN M. SANDOVAL M.; FLORES, GABRIELA V. ARAUJO V.; FERREIRA, AUREA F. F.; DA MATTA, VANIA L. R.; GOMES, CLAUDIA M. DE CASTRO M.; SOSA-OCHOA, WILFREDO H. H.; ZUNIGA, CONCEPCION; SILVEIRA, FERNANDO T. T.; CORBETT, CARLOS E. P.; LAURENTI, MARCIA D.. Role of antigen-presenting cells in non-ulcerated skin lesions caused by Leishmania (Leishmania) infantum chagasi. PARASITE IMMUNOLOGY, v. N/A, p. 11-pg., 2023-01-31. (18/04698-6, 17/24834-9, 14/50315-0)

FLORES, GABRIELA V. ARAUJO; PACHECO, CARMEN M. SANDOVAL; FERREIRA, AUREA F.; TOMOKANE, THAISE YUMIE; NUNES, JULIANA B.; COLOMBO, FABIO A.; SOSA-OCHOA, WILFREDO H.; ZUNIGA, CONCEPCION; SILVEIRA, FERNANDO T.; CORBETT, CARLOS E. P.; et al. Leishmania (L.) infantum chagasi isolated from skin lesions of patients affected by non-ulcerated cutaneous leishmaniasis lead to visceral lesion in hamsters. Parasitology International, v. 93, p. 9-pg., 2022-12-26. (17/24834-9, 18/04698-6, 14/50315-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

PACHECO, Carmen Maria Sandoval. Non-ulcerated or atypical cutaneous leishmaniasis caused by L. (L.) infantum chagasi in Amapala municipality, Valle, Honduras: immunohistopathological characterization of the skin lesions with emphasis to the involvement of the antigen presentation cells. 2022. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) São Paulo.