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Non-ulcerated or atipycal Cutaneous Leishmaniasis caused by Leishmania (L.) infantum chagasi in the municipality of Amapala, Valle, Honduras: immunohistopathological characterization of skin lesions with emphasis in the participation of antigen presenting cells

Grant number: 18/04698-6
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2019
Effective date (End): August 31, 2021
Field of knowledge:Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
Principal Investigator:Marcia Dalastra Laurenti
Grantee:Carmen Maria Sandoval Pacheco
Home Institution: Faculdade de Medicina (FM). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:14/50315-0 - Leishmaniasis in Latin America: an advanced perspective on immunopathogenetic factors of cutaneous and visceral infection, immunomodulators of the sandflies vector saliva and immunogenic exo-antigens of Leishmania (L.) infantum chagasi as vaccine candidates, AP.TEM

Abstract

In the Americas, especially in South America, infection with Leishmania (Leishmania) infantum chagasi causes subclinical manifestations and visceral leishmaniasis (LV), which when untreated is potentially fatal. In Honduras, infection by Leishmania (L.) infantum chagasi causes Visceral Leishmaniasis (VL), but non-ulcerated or atypical cutaneous leishmaniasis (LCNU) caused by this parasite species has also been reported. Cases of non-ulcerated or atypical cutaneous leishmaniasis (NUCL) have been reported, also in other countries of the region, such as Costa Rica, El Salvador and Nicaragua. In Honduras, there are well-identified endemic areas of this non-ulcerated clinical variant, located in the departments of Choluteca and Valle, as well as southern parts of the departments of Francisco Morazán, La Paz, El Paraíso and Intibucá. In this study, 20 skin biopsies will be used in the Laboratory of Pathology of Infectious Diseases of FMUSP, which have already been collected for the development of another project. After clinical and laboratory examination to confirm Leishmania infection, biopsies of skin lesions of these patients, which are defined as a painless, non-ulcerative papule, infiltrative plaque or nodule, erythematous or skin color, with or without the presence of halo hypo-pigmented, were collected, fixed in 10% buffered formol solution 0.01M and processed by the usual techniques of optical microscopy. Histological sections of the cutaneous lesions will be processed by the immunohistochemical reaction using the primary antibodies to Langerhans cell markers (CD1a, CD207) and dermal dendritic cells (CD11c, CD123, factor XIIIa); M1 macrophages (CD68) and M2 macrophages (CD163); and double-stain immunohistochemistry using iNOS markers and production of cytokines such as IL-12, TNF-±, IL-4, IL-10, TGF-² and IL-13. (AU)