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Genomic sequencing of frog virus 3 isolated from American bullfrog (Lithobates catesbeianus) reared in ranaculture in Brazil

Grant number: 19/02075-4
Support type:Scholarships abroad - Research
Effective date (Start): July 01, 2019
Effective date (End): August 01, 2019
Field of knowledge:Agronomical Sciences - Fishery Resources and Fishery Engineering
Principal Investigator:Cláudia Maris Ferreira Mostério
Grantee:Cláudia Maris Ferreira Mostério
Host: Thomas B Waltzek
Home Institution: Instituto de Pesca. Agência Paulista de Tecnologia dos Agronegócios (APTA). Secretaria de Agricultura e Abastecimento (São Paulo - Estado). São Paulo , SP, Brazil
Local de pesquisa : University of Florida, Gainesville (UF), United States  

Abstract

Ranaviruses are emerging pathogens that target amphibians, reptiles, and fish. In addition to their lethality virus of this genus can be transmitted between these three classes of vertebrates. Some strains have been shown to be more virulent than others and may have significant differences in genes associated with virulence. For the understanding of the circulation of this pathogen in aquatic systems and its dynamics of dissemination in Brazil, sequencing of the complete genome of different strains is highly recommended. Thus, the objective of this study is to perform the genomic sequencing of ranavirus Frog virus 3 (FV3) in American bullfrogs (Lithobates catesbeianus) from raniculture in Brazil and to determine its phylogenetic relationship with other strains already described in the literature. FV3 positive samples obtained from adult bullfrogs from a frog farm situated in the city of Tapiratiba/SP will be used. These samples were isolated in cell culture and sent to the Wildlife and Aquatic Veterinary Disease Laboratory on the University of Florida for complete sequencing of the viral genome, after appropriate authorizations. Sequencing and phylogenetic analysis will be done by the BLAST program and other software described and validated in the international literature. This procedure and consequent knowledge of the virulence genes of FV3 are essential for the understanding and eradication of ranavirus in Brazil, collaborating not only with the preservation of our biodiversity but also with the productive sector of aquaculture bringing economic gains to this area of Brazilian agribusiness.