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Characterization of the interrelationship between transcriptomes, miRNomes and proteomes from the venom glands of Bothrops fonsecai and Bothrops cotiara

Grant number: 18/26520-4
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): March 01, 2019
Effective date (End): February 28, 2021
Field of knowledge:Biological Sciences - Genetics
Cooperation agreement: NSF - Dimensions of Biodiversity and BIOTA
Principal Investigator:Inácio de Loiola Meirelles Junqueira de Azevedo
Grantee:Pedro Gabriel Nachtigall
Home Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
Associated research grant:16/50127-5 - Dimensions US-BIOTA São Paulo: scales of biodiversity: integrated studies of snake venom evolution and function across multiple levels of diversity, AP.BTA.TEM

Abstract

Differences in venom composition produced by different snake species can result from distinct regulatory mechanisms occurring in each species. It is known that changes in expression levels of components of genetic regulatory networks are responsible for phenotypic differences. Recently, few studies showed that miRNAs can be related to modulation of the differential venom composition in ontogenetic shifting in adults and juveniles of snakes belonging to the Genus Crotalus and Hydrophis. Then, all these data together suggest that post-transcriptional modulation play key roles on venom composition and production. Despite these studies, comparative analysis among species focusing to identify regulatory elements and patterns that led to venom composition are still scarce. A study using proteome analysis reported differences in venom composition in Bothrops fonsecai and B.cotiara, despite the genetic similarity due to the recent divergence time between both species. In this sense, we will integrate data from transcriptome, miRNome and proteome analysis of B. fonsecai and B. cotiara, to identify miRNAs and their binding sites related to the differences in venom composition in snake species. Additionally, we will also check for putative elements in 5'UTR, such as uORFs, that can be involved in the increasing or decreasing of specific protein levels. The comparative analysis will help to bring insights into the modulatory components of the GRNs that shape the venom production in snakes.