Study of the post-translational mechanisms involved on increased nitric oxide bioavailability in spotaneoulsy hypertensive rats treated with apocynin

• Grant number: 17/18436-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2019
• End date: August 31, 2021
• Field of knowledge: Biological Sciences - Pharmacology - Cardiorenal Pharmacology
• Principal Investigator: Cristina Antoniali Silva
• Grantee: Murilo Eduardo Graton
• Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil
• Associated scholarship(s): 19/13751-0 - Role of histones on cardiac dysfunction in sepsis-induced cardiomyopathy, BE.EP.DR

Abstract

Nitric Oxide (NO) is the major vasodilator molecule in the body and it is synthesized by the NO synthase enzymes, and the isoform III or endothelial (eNOS) is the main responsible for the production of NO in the blood vessels. Increased production of Reactive Oxygen Species (ROS) by oxidative enzymes such as NOX enzymes can lead to oxidative stress and reduce NO bioavailability. We have recently shown that apocynin, a NOX inhibitor, is able to increase eNOS expression and activity and NO bioavailability in spontaneously hypertensive rats (SHR). The objective of this work will be to evaluate the possible changes in post-translational modification mechanisms that control eNOS and the bioavailability of NO in SHR treated with apocynin (30 mg / kg, p.o.). Western Blotting assays will be performed for the evaluation of the protein expression of eNOS, calmodulin, caveolin-1, Hsp-90, Nrf-2, catalase, superoxide dismutase and glutathione peroxidase, as well as their interactions through co-immunoprecipitation. Functional studies of vascular reactivity in mesenteric artery rings to evaluate the participation of Hsp-90 and eNOS in vasoconstriction induced by phenylephrine. The intracellular concentrations of hydrogen peroxide will be quantified by flow cytometry. The results obtained will be expressed as mean ± SEM and statistically analyzed by statistical analysis of one-way ANOVA or Student's T Test and a significance level of 5% (p <0.05) will be adopted. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GRATON, MURILO E.; FERREIRA, BRUNO H. S. H.; TROIANO, JESSICA A.; POTJE, SIMONE. R. R.; VALE, GABRIEL. T. T.; NAKAMUNE, ANA CLAUDIA M. S.; TIRAPELLI, CARLOS R.; MILLER, FRANCIS. J. J.; XIMENES, VALDECIR. F. F.; ANTONIALI, CRISTINA. Comparative study between apocynin and protocatechuic acid regarding antioxidant capacity and vascular effects. FRONTIERS IN PHYSIOLOGY, v. 13, p. 14-pg., 2022-11-15. (16/22180-9, 16/01476-7, 17/18436-0, 14/50926-0)

GRATON, MURILO; BENEVIDES, PRISCILA SCARPIM; TROIANO, JESSICA ANTONINI; POTJE, SIMONE REGINA; GONCALVES, EMILY; ZANARDO, JESSICA; NAKAMUNE, ANA CLAUDIA; ANTONIALI, CRISTINA. Antioxidant Defense in Mesenteric Arteries from Spontaneously Hypertensive Rats Treated with Apocynin. Free Radical Biology and Medicine, v. 145, p. 1-pg., 2019-12-01. (19/13751-0, 16/22180-9, 17/18436-0)

BARROSO, HELENA C.; GRATON, MURILO E.; POTJE, SIMONE R.; TROIANO, JESSICA A.; SILVA, LILIAN X.; NAKAMUNE, ANA CLAUDIA M. S.; ANTONIALI, CRISTINA. Data of Nebivolol on oxidative stress parameters in hypertensive patients. DATA IN BRIEF, v. 41, p. 11-pg., 2022-02-11. (16/22180-9, 17/18436-0, 15/09373-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GRATON, Murilo Eduardo. Role of the chronic treatment with apocynin on the nitric oxide bioavailability and the reactive oxygen species generation in spontaneously hypertensive rats. 2022. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.