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Development and validation of a combined LC-MS/MS assay for determination of sirolimus, everolimus, tacrolimus and ciclosporin in whole blood.

Grant number: 19/03905-0
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): July 01, 2019
Effective date (End): June 30, 2020
Field of knowledge:Biological Sciences - Pharmacology - General Pharmacology
Principal researcher:Gilberto de Nucci
Grantee:Natalícia de Jesus Antunes
Supervisor abroad: Atholl Christopher Johnston
Home Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Research place: Queen Mary University of London, England  
Associated to the scholarship:16/22506-1 - Metabolism of dapaconazole, BP.PD

Abstract

In therapy with immunosuppressant drugs, therapeutic drug monitoring (TDM) is essential to treatment success. Therefore, analytical methods with higher precision, accuracy, sensitivity, selectivity and speed are demanding. This project aims to develop an improved sample-preparation protocol for simultaneous quantification of sirolimus, everolimus, tacrolimus and ciclosporin in whole blood by ultra high performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS). The sample preparation will be performed by protein precipitation with ZnSO4 and acetonitrile, and liquid-liquid extraction using methanol. The drugs separation will be performed with a Waters UPLC BEH C18 (50 mm × 2.1 mm) 1.7-m column maintained at 45 æC using the mobile phase A consisted of 2 mM ammonium acetate with 0.1% formic acid (v/v) in water, and mobile phase B consisted of 2 mM ammonium acetate with 0.1% formic acid (v/v) in methanol. The mass spectrometer will operate in multiple reaction monitoring (MRM) in positive electrospray ionization mode. The method validation will be performed according to European Medicines Agency validation guideline. Comparison of the results obtained with the previous routine LC-MS/MS method and new rapid LC-MS/MS method will be done using a set of proficiency testing samples and real patient samples.

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