Gene expression profile of LHb in the pituitary of Astyanax altiparanae females, induced with synthetic hormones, using real-time quantitative polymerase chain reaction (RT-qPCR)

• Grant number: 19/05741-5
• Support type: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): May 15, 2019
• Effective date (End): October 28, 2019
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Sergio Ricardo Batlouni
• Grantee: Mariana Roza de Abreu
• Supervisor abroad: Julien Bobe
• Home Institution: Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Local de pesquisa : Institut National de la Recherche Agronomique, Rennes (INRA), France
• Associated to the scholarship: 16/25988-7 - Induction to final maturation and ovulation of lambari (Astyanax altiparanae) with salmon GnRH analogue, BP.DR

Abstract

In our recent experiments we have evaluated the use of synthetic hormones in order to improve the reproductive performance of Astyanax altiparanae females. Our hypothesis was the GnRH analogues could stimulate the luteinizing hormone (LH) - 17a,20b-dihydroxy-4-pregnen-3-one (17,20b-P) - prostaglandin F2a (PGF2a) axis and provoke final oocytes maturation and ovulation. As GnRHa play a role to trigger the pituitary to synthetize and release LH, it is very important to characterize the gene expression profile of lhb in the pituitary to understand how different doses of these synthetic hormones act in this part of the hypothalamus-pituitary-gonad axis of this species. Thus, the aim of this project is to investigate the gene expression profile of lhb in the pituitary of A. altiparanae using real time quantitative polymerase chain reaction (qPCR). For that, will be used pituitary glands from A. altiparanae females collected on experiments carried out in Brazil. In these experiments a wide range of doses of [D-Arg6, Pro9, NEt]-sGnRH and [des-Gly10, D-Ala6]-LHRH alone or in association with domperidone was applied. Pituitaries were collected before treatment to represent the baseline conditions and after spawning, which represented the final collection. The samples were stored in RNAlater at -80°C. The RNA samples will be extracted using the RNAaqueous Micro Kit (Invitrogen). For cDNA cloning, the reverse transcription will be performed of individual pituitaries in combination with Superscript III and primers, according to the manufacturer's instructions (Invitrogen). The qPCR will be performed at the Fish Physiology and Genomics Institute - INRA in France. The results will be discussed under the assistance of supervisor, Prof. Dr. Julien Bobe, who is known expert in fish reproductive biology and genomics. This internship opportunity allows the establishment of an international partnership with the Prof. Dr. Julien Bobe and will improve the scientific perspective and the network of the PhD student. Also, the PhD student will have the knowledge of new techniques and will be able to transfer this knowledge to Aquaculture Center and to Brazilian collaborators being able to start a new field of research.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ROZA DE ABREU, MARIANA; SILVA, LAIZA MARIA DE JESUS; FIGUEIREDO-ARIKI, DANIEL GUIMARAES; SATO, RAFAEL TOMODA; KURADOMI, RAFAEL YUTAKA; BATLOUNI, SERGIO RICARDO. Reproductive performance of lambari (Astyanax altiparanae) in a seminatural system using different protocols. AQUACULTURE RESEARCH, v. 52, n. 2, p. 471-483, FEB 2021. Web of Science Citations: 0.