In vitro celularization of oxidized cellulose for skin repair

• Grant number: 18/26539-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: April 01, 2019
• End date: May 31, 2020
• Field of knowledge: Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
• Principal Investigator: Eliane Trovatti
• Grantee: Victória Maria Hebling
• Host Institution: Universidade de Araraquara (UNIARA). Araraquara , SP, Brazil

Abstract

Accidents that lead to loss of cutaneous tissue are frequent and depending on the extent, can lead to death by dehydration. Medical measures for immediate containment of the problem are removal of tissue residues and covering the injured area with dressings based on hydrogels to avoid contamination and loss of water. Among the biomaterials available for use in tissue engineering polymers, more specifically the natural polymers, represent a class of special interest because of their properties. Cellulose is a natural polymer composed only of glucose monomers that has recently received great recognition in biomedical applications due to its characteristics such as biocompatibility, absence of cytotoxicity, hydrophilicity, good water retention capacity, among others. Thus, the objective of this project is the development of membranes based on chemically modified cellulose and the analysis of their potential in the in vitro cutaneous regeneration process studies. For this, this hydrogel will be used for the preparation of wet and dry membranes. The membranes will be cellularled with human fibroblasts to test the behavior of these cells in the presence of this biomaterial, through cytotoxicity tests and cell adhesion in vitro.