Scaffolds composed by bioactive glasses and injectable matrices for bone regeneration

• Grant number: 18/25871-8
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: April 01, 2019
• End date: March 31, 2024
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Physical-Chemistry
• Principal Investigator: Ana Paula Ramos
• Grantee: Lucas Fabrício Bahia Nogueira
• Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 22/01219-5 - Development of 3D cell-free biomimetic scaffold to support endochondral ossification, BE.EP.DR

Abstract

Design of three-dimensional hybrid and injectable templates (scaffolds) has emerged as a promisor approach to produce biomaterials for targeted applications. In this sense, the properties of the materials should be tuned to improve its therapeutic effect in the host tissue and to become a material able to easily adapt and shape to any type of bone defect. On that account, the use of bioactive glasses, or bioglass (BG) in the composition of these scaffolds may improve their osteogenic activity due to the capability of these vitreous systems to interact with the bone driven by chemical and biochemical processes stimulated by the dissolution products of the BG. So, the objective of this project is to synthesize and characterize scaffolds constituted either by BG SiO2-CaO-P2O5 / SiO2-SrO-P2O5, containing Eu3+ and modified by the addition of Ta5+ or Nb5+, or by the association of these vitreous systems with matrices based on gelling properties of chitosan (Ch) and k-carrageenan (k-Carr). Effects on the mechanical, surface, bioactive and biological properties of these three-dimensional systems promoted by both the modifications in the composition of the vitreous systems and the combinations of these BG with injectable hydrogels will be investigated. Furthermore, it is proposed an in vitro study aimed at monitoring the biodegradation products of these systems in the tissues and organs by the presence of the biomarking property conferred by the incorporation of europium Eu3+. For the synthesis of these vitreous systems, sol-gel methodologies will be adopted, and later, to obtain the hybrid scaffolds, the BG will be added together with crosslinking agents to solutions containing the polysaccharides chosen in this project for the occurrence of in situ gelation. Posteriorly, both the BG and the scaffolds will be characterized with respect to their composition using infrared vibration spectroscopy (FTIR), X-ray diffraction (XRD) and Raman scattering. The morphology and porosity will be evaluated by the scanning electron microscopy and X-ray computed microtomography (micro-CT). Bioactivity and biodegradation will be studied by immersing the samples obtained in the body fluid that simulates the pH and the ionic concentration of the blood plasma (SBF, simulated body fluid). Evaluation of the luminescent properties of systems containing Eu3+ in vitro will occur by the photoluminescence spectroscopy. The strength of the material will be evaluated by mechanical tests, in which the resistance will be given by Young's modulus analysis. After, it will be characterized with respect to surface energy and wettability using contact angle measurements. The best results regarding composition, morphology, strength and surface properties will be selected for osteoblast culture tests in vitro. It is expected at the end of this project that the produced scaffolds can act, as well as bone regenerating systems, as carriers of compounds with pharmacological activity, such as curcumin, so that, through its biodegradation, both the controlled release in situ of this compound as a stimulus for the growth of the new tissue that should fill the site of the bone defect.

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NOGUEIRA, LUCAS FABRICIO BAHIA; EUFRASIO CRUZ, MARCOS ANTONIO; AGUILAR, GUILHERME JOSE; TAPIA-BLACIDO, DELIA RITA; DA SILVA FERREIRA, MARCIA ELIANA; MANIGLIA, BIANCA CHIEREGATO; BOTTINI, MASSIMO; CIANCAGLINI, PIETRO; RAMOS, ANA PAULA. Synthesis of Antibacterial Hybrid Hydroxyapatite/Collagen/Polysaccharide Bioactive Membranes and Their Effect on Osteoblast Culture. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 23, n. 13, p. 22-pg., 2022-07-01. (18/25871-8, 20/08727-0, 16/25955-1, 19/25054-2, 21/05947-2)

NOGUEIRA, LUCAS FABRICIO BAHIA; MANIGLIA, BIANCA C.; BUCHET, RENE; MILLAN, JOSE LUIS; CIANCAGLINI, PIETRO; BOTTINI, MASSIMO; RAMOS, ANA PAULA. hree-dimensional cell-laden collagen scaffolds: From biochemistry to bone bioengineerin. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 110, n. 4, NOV 2021. (19/08568-2, 20/08727-0, 19/25054-2, 18/25871-8)

NOGUEIRA, LUCAS FABRICIO BAHIA; CRUZ, MARCOS ANTONIO EUFRASIO; TOVANI, CAMILA BUSSOLA; LOPES, HELENA BACHA; BELOTI, MARCIO MATEUS; CIANCAGLINI, PIETRO; BOTTINI, MASSIMO; RAMOS, ANA PAULA. Curcumin-loaded carrageenan nanoparticles: Fabrication, characterization, and assessment of the effects on osteoblasts mineralization. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 217, p. 9-pg., 2022-09-01. (19/25054-2, 19/08568-2, 18/25871-8)

NOGUEIRA, LUCAS FABRICIO BAHIA; CRUZ, MARCOS ANTONIO EUFRASIO; MELO, MARYANNE TRAFANI DE; MANIGLIA, BIANCA CHIEREGATO; CAROLEO, FABRIZIO; PAOLESSE, ROBERTO; LOPES, HELENA BACHA; BELOTI, MARCIO M.; CIANCAGLINI, PIETRO; RAMOS, ANA PAULA; et al. Collagen/Kappa-Carrageenan-Based Scaffolds as Biomimetic Constructs for In Vitro Bone Mineralization Studies. Biomacromolecules, v. 24, n. 3, p. 9-pg., 2023-02-14. (19/08568-2, 20/08727-0, 22/01219-5, 19/25054-2, 16/25955-1, 18/25871-8, 21/05947-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

NOGUEIRA, Lucas Fabrício Bahia. Designing biomimetic polymer scaffolds for bone mineralization investigation. 2024. Doctoral Thesis - Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (PCARP/BC) Ribeirão Preto.