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Chronic effect of blood orange juice intake on microRNA expression profile and inflammatory response in overweight women

Grant number: 18/25046-7
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): May 01, 2019
Effective date (End): November 30, 2020
Field of knowledge:Health Sciences - Nutrition - Nutrition Biochemistry
Principal Investigator:Marcelo Macedo Rogero
Grantee:Vinícius Cooper Capetini
Home Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:13/07914-8 - FoRC - Food Research Center, AP.CEPID
Associated scholarship(s):19/23182-3 - Analysis of metabolites Phase-2 of bioactive compounds of blood orange (Citrus sinensis (L.) Osbeck) juice in plasma of overweight women, BE.EP.DR

Abstract

The western dietary pattern is related to the increase in the plasma concentration of pro-inflammatory biomarkers that lead to the development of methylation. Fruit consumption - through the action of different bioactive compounds - represents a nutritional strategy with the potential to modulate the inflammatory response related to the pathophysiology of different metabolic diseases. Recent studies suggest that microRNA acts as inflammatory biomarkers and the analysis of its expression may contribute to the identification of the risk of chronic non-communicable diseases. The screen project aims to investigate the effect of chronic intake of blood orange juice on the microRNA profile and the inflammatory response in overweight women. An intervention study will be performed with women (n = 20) between 18 and 40 years of age, diagnosed as being overweight (Body Mass Index (BMI) equal to 25 - 29.9 kg/m2). During 4 weeks, volunteers will ingest 500 mL of blood orange juice daily with blood sampling at baseline and 2 and 4 weeks after beginning drinking. Anthropometric evaluation, blood pressure measurement, caloric intake, flavonoid and vitamin C calculation, lipid profile, determination of blood glucose concentrations, insulin, lipopolysaccharide binding protein, soluble differentiation cluster, amylase, fibrinogen, D-dimer, urea, creatinine, gamma glutamyl transferase, alanine aminotransferase and aspartate aminotransferase, bioactive compounds and inflammatory biomarkers [C-reactive protein, interleukin (IL) -6, IL-10, tumor necrosis factor alpha (TNF- ), monocyte-1 chemotactic protein and adiponectin]. Evaluation of microRNA expression will be performed on plasma samples and peripheral blood mononuclear cells. The expression of TNF-± genes, nuclear transcription factor kappa B (NF-ºB), nuclear transcription factor inhibitor kappa B alpha (IºB-±), IL1-beta, IL-6, IL-10 , toll-type receptor (TLR) -2 and TLR-4. In addition to these assays, the content and phosphorylation of NF-ºB, IºB±, c-jun N-terminal kinase 1 protein kinase, IºB-± inhibitor kinase and beta 1 growth-transforming factor will be evaluated. (AU)