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In vitro cellular reprogramming to pluripotency (IPS) in the swine model from non-transgenic and non-invasive methodology

Grant number: 19/02811-2
Support type:Scholarships in Brazil - Master
Effective date (Start): April 01, 2019
Effective date (End): January 31, 2021
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:Fabiana Fernandes Bressan
Grantee:Kaiana Recchia
Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Associated research grant:15/26818-5 - Investigation of cellular and molecular mechanisms on in vitro induced toti- and pluripotency acquisition - a translational approach, AP.JP

Abstract

Embryonic stem cells (ESC) are able to differentiate in the three embryonic leaflets due to their undifferentiated state, but their collection promotes on the embryo (blastocyst) infeasible and consequently faces ethical problems in the human model. However, with the possibility of inducing a differentiated cell to an undifferentiated state through the expression of embryonic pluripotency factors, it was possible to produce induced pluripotent cells (iPS), being interesting for regenerative medicine and cell therapy due to its high potential in both veterinary medicine as well as human. However, with the possibility of inducing a differentiated cell to an undifferentiated state through the expression of embryonic pluripotency factors, it was possible to produce induced pluripotent cells (iPS), being interesting for regenerative medicine and cell therapy due to its high potential in both veterinary medicine as well as human. O estudo do modelo suíno torna-se mais interessante devido a sua maior compatibilidade e viabilidade com o modelo humano, sendo semelhanças fisiológicas, morfológicas, sistema imunológico, entre outros. Consequently to the possibility of induction of pluripotent cells through the STEMCCA vector and of episomal vectors, in this study we intend to generate transgenic and non-transgenic iPS cells, respectively, from three different tissues and collect, being: fibroblasts from skin biopsy cells blood mononuclear cells from peripheral blood collection and progenitor cells from collecting urine from the swine model. To perform the characterization of the iPS cells obtained from the different tissues and collections by the alkaline phosphatase test, morphology and in vitro differentiation, for later analysis of the results and comparison of the efficiency of the cells and evaluating the possibility of working with genetic engineering with focus in regenerative and translational medicine. From the results obtained in conjunction with other results of the projects developed in the laboratory, these will be used as a basis for future differentiation experiment.