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In vitro effects of osteoblast-derived osteopontin on autophagic flux in human oral squamous cell carcinoma cells

Grant number: 19/04282-7
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2019
Effective date (End): April 30, 2020
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Lucas Novaes Teixeira
Grantee:Pedro Keese de Castro
Home Institution: Centro de Pesquisas Odontológicas São Leopoldo Mandic. Faculdade São Leopoldo Mandic (SLMANDIC). Sociedade Regional de Ensino e Saúde S/S Ltda (SRES). Campinas , SP, Brazil

Abstract

Squamous cell carcinoma (SCC) is the most prevalent malignant neoplasm of the oral structures, which may invade and destroy bone tissue due to increased osteoclastic activity. The matricelular protein osteopontin (OPN) has been associated with more aggressive tumors, since OPN can promote cell adhesion, proliferation and invasion. In bone, OPN is the most abundant non-collagenous protein, especially concentrated at bone interfaces (cement lines and the lamina limitantes), and play important roles in osteoblast and osteoclast adhesion and function. Teixeira et al. (2016) have demonstrated that osteoblast-derived OPN affects the interactions among oral SCC-derived epithelial cells, osteoblasts, and osteoclasts, which could contribute to the process of bone destruction during bone invasion by oral SCC. The role of osteoblast-derived OPN on autophagy process in oral SCC cells, however, is not fully understood. In this context, the present study aims to evaluate the effects of osteoblast-derived OPN in cocultures of osteoblastic cells and oral SCC and its effects on the autophagy process in oral SCC. In this context, the aim of the present will be to evaluate the autophagic flux by means of p62 e LC3 quantification by western blotting in oral SCC cells cocultured with osteoblastic cells.