Development of electrochemical and colorimetric biosensors for detecting micotoxins

• Grant number: 17/21791-7
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: May 01, 2019
• End date: March 31, 2022
• Field of knowledge: Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
• Principal Investigator: Daniel Souza Corrêa
• Grantee: Fernanda Lanzoni Migliorini
• Host Institution: Embrapa Instrumentação Agropecuária. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
• Associated research grant: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis, AP.TEM

Abstract

The present project aims at the development of electrochemical and colorimetricsensors, based on nanofibers modified with nanomaterials that can aid and improve thedetection of mycotoxins. Mycotoxins are secondary metabolites produced by a largenumber of fungi. Among mycotoxins, aflatoxins (FA) are considered to be the mosttoxicogenic fungal metabolites with carcinogenic, mutagenic, teratogenic andimmunosuppressive effects. The aim of this project is to develop accurate and costeffective sensors using polymer nanofibers modified with distinct nanomaterials, aimingat aflatoxins detection. The sensorial nanometric platforms will be characterizedthrough experimental techniques including atomic force microscopy (AFM), ScanningElectron Microscopy (SEM), Thermal Analysis (DSC and TGA) and InfraredSpectroscopy (FTIR). Electrochemical detection will be carried out usingelectrochemical techniques, i.e., cyclic voltammetry , squarewave voltammetry , andamperometric in standard solutions, and colorimetric detection will be performed usingthe UV-Vis absorption and fluorescence spectroscopy technique. Subsequently, bothdeveloped sensors will be tested on actual samples.

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MERCANTE, LUIZA A.; PAVINATTO, ADRIANA; PEREIRA, TAMIRES S.; MIGLIORINI, FERNANDA L.; DOS SANTOS, DANILO M.; CORREA, DANIEL S.. Nanofibers interfaces for biosensing: Design and applications. SENSORS AND ACTUATORS REPORTS, v. 3, NOV 2021. (17/20973-4, 18/22214-6, 17/21791-7, 20/10048-4)

SOARES, ANDREY COATRINI; SOARES, JULIANA COATRINI; DOS SANTOS, DANILO MARTINS; MIGLIORINI, FERNANDA L.; POPOLIN-NETO, MARIO; PINTO, DANIELLE DOS SANTOS CINELLI; CARVALHO, WANESSA ARAUJO; BRANDAO, HUMBERTO MELLO; PAULOVICH, FERNANDO VIEIRA; CORREA, DANIEL SOUZA; et al. Nanoarchitectonic E-Tongue of Electrospun Zein/Curcumin Carbon Dots for Detecting Staphylococcus aureusin Milk. ACS OMEGA, v. 8, n. 15, p. 12-pg., 2023-04-04. (17/20973-4, 18/22214-6, 18/18953-8, 17/21791-7)

TEODORO, KELCILENE B. R.; SANFELICE, RAFAELA C.; MIGLIORINI, FERNANDA L.; PAVINATTO, ADRIANA; FACURE, MURILO H. M.; CORREA, DANIEL S.. A Review on the Role and Performance of Cellulose Nanomaterials in Sensors. ACS SENSORS, v. 6, n. 7, p. 2473-2496, JUL 23 2021. (18/22214-6, 17/21791-7, 17/19470-8, 17/12174-4, 17/10582-8)

MIGLIORINI, FERNANDA L.; TEODORO, KELCILENE B. R.; CORREA, DANIEL S.. GREEN-SYNTHESIZED GOLD NANOPARTICLES SUPPORTED ON CELLULOSE NANOWHISKERS FOR EASY-TO-INTERPRET COLORIMETRIC DETECTION OF CADMIUM (II). CELLULOSE CHEMISTRY AND TECHNOLOGY, v. 54, n. 5-6, p. 407-413, MAY-JUN 2020. (17/21791-7, 18/09414-6, 14/21184-5, 17/12174-4)

SANTOS, DANILO M. DOS; CARDOSO, RAFAEL M.; MIGLIORINI, FERNANDA L.; FACURE, MURILO H. M.; MERCANTE, LUIZA A.; MATTOSO, LUIZ H. C.; CORREA, DANIEL S.. Advances in 3D printed sensors for food analysis. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 154, p. 20-pg., 2022-09-01. (17/10582-8, 17/20973-4, 18/22214-6, 20/14946-7, 17/21791-7)

DOS SANTOS, DANILO M.; MIGLIORINI, FERNANDA L.; SOARES, ANDREY C.; MATTOSO, LUIZ H. C.; OLIVEIRA, OSVALDO N., JR.; CORREA, DANIEL S.. Electrochemical Immunosensor Made with Zein-based Nanofibers for On-site Detection of Aflatoxin B1. Electroanalysis, v. N/A, p. 9-pg., 2022-06-15. (17/12174-4, 18/18953-8, 17/20973-4, 18/22214-6, 20/14946-7, 17/21791-7)

UEHARA, THIERS M.; MIGLIORINI, FERNANDA L.; FACURE, MURILO H. M.; PALMA FILHO, NICOLAU B.; MIRANDA, PAULO B.; ZUCOLOTTO, VALTENCIR; CORREA, DANIEL S.. anostructured scaffolds containing graphene oxide for nanomedicine application. POLYMERS FOR ADVANCED TECHNOLOGIES, v. 33, n. 2, NOV 2021. (17/12174-4, 17/10582-8, 18/02819-0, 17/21791-7, 18/22214-6)

CARDOSO, RAFAEL M.; PEREIRA, TAMIRES S.; DOS SANTOS, DANILO M.; MIGLIORINI, FERNANDA L.; MATTOSO, LUIZ H. C.; CORREA, DANIEL S.. Laser-induced graphitized electrodes enabled by a 3D printer/diode laser setup for voltammetric detection of hormones. Electrochimica Acta, v. 442, p. 8-pg., 2023-01-18. (18/22214-6, 17/20973-4, 17/21791-7, 20/14946-7)

MIGLIORINI, FERNANDA L.; DOS SANTOS, DANILO M.; SOARES, ANDREY C.; MATTOSO, LUIZ H. C.; OLIVEIRA JR, OSVALDO N.; CORREA, DANIEL S.. Design of A Low-Cost and Disposable Paper-Based Immunosensor for the Rapid and Sensitive Detection of Aflatoxin B1. CHEMOSENSORS, v. 8, n. 3, p. 14-pg., 2020-09-01. (17/20973-4, 18/22214-6, 18/18953-8, 17/21791-7)