Integrated metabolism analysis for Glycine max responses to water stress

• Grant number: 19/08477-7
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• Start date: August 01, 2019
• End date: July 31, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Norberto Peporine Lopes
• Grantee: Paula Carolina Pires Bueno
• Supervisor: Joachim Kopka
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: Max Planck Society, Potsdam, Germany
• Associated to the scholarship: 17/19702-6 - Integrated omics approaches for the evaluation of the micromolecular phenotypic dynamics of Glycine max (soja) genetically modified for water stress tolerance, BP.PD

Abstract

Soybean (Glycine max, Leguminosae) is one of the most important food crops in the world, since the grains are primary and rich source of vegetable oil and protein for human and animal feed. However, soybean productivity is highly impacted by events such as drought and flooding. Indeed, abiotic stresses cause serious losts in many food crops wordlwide. They are directly related to changes in several cellular metabolic pathways, such as carbohydrates, amino acid and peptide metabolism. If the metabolic homeostasis is disrupted, there might be a reduction in growth, development and, consequently, yield. Considering this susceptibility, many efforts have been made aiming the development and characterization of more tolerant plant genotypes, along with the establishment of suitable approaches for the identification and annotation of molecular traits that vary in response to these events. In plant breeding, genomics and transcriptomics have been extensively used for the development and characterization of improved crops. However, the identification and annotation of molecular traits that vary in response to stress events remain a challenge. Therefore, the present study aims the identification and quantitation of water stress-responsive specialized metabolites from different soybean genotypes (two of them genetically modified with the gene AtGolS2 for galactinol synthase - GolS), cultivated under controlled, drought and flooding conditions, as well as to integrate this information with previously obtained data concerning the secondary metabolism and transcriptomics in order to understand the role of plant adaptations to water stresss.

Scientific publications

(6)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

REGINALDO, FERNANDA PRISCILA SANTOS; BUENO, PAULA CAROLINA PIRES; LOURENCO, ESTELA MARIANA GUIMARAES; DE MATOS COSTA, ISABELLY CRISTINA; MOREIRA, LETICIA GONDIM LAMBERT; DE ARAUJO ROQUE, ALAN; BARBOSA, EUZEBIO GUIMARAES; FETT-NETO, ARTHUR GERMANO; CAVALHEIRO, ALBERTO JOSE; GIORDANI, RAQUEL BRANDT. Methyl jasmonate induces selaginellin accumulation in Selaginella convoluta. METABOLOMICS, v. 19, n. 1, p. 13-pg., 2022-12-21. (14/50926-0, 19/08477-7, 17/19702-6)

SANTOS REGINALDO, FERNANDA PRISCILA; PIRES BUENO, PAULA CAROLINA; DE MATOS COSTA, ISABELLY CRISTINA; ROQUE, ALAN DE ARAUJO; FETT-NETO, ARTHUR GERMANO; CAVALHEIRO, ALBERTO JOSE; GIORDANI, RAQUEL BRANDT. Molecular Networking Discloses the Chemical Diversity of Flavonoids and Selaginellins in Selaginella convoluta. Planta Medica, v. 87, n. 01/02, p. 113-123, FEB 2021. (14/50926-0, 19/08477-7, 17/19702-6)

BUENO, PAULA C. P.; LOPES, NORBERTO P.. Metabolomics to Characterize Adaptive and Signaling Responses in Legume Crops under Abiotic Stresses. ACS OMEGA, v. 5, n. 4, p. 1752-1763, FEB 4 2020. (14/50265-3, 17/19702-6, 19/08477-7)

MANNOCHIO-RUSSO, HELENA; BUENO, PAULA CAROLINA P.; BAUERMEISTER, ANELIZE; DE ALMEIDA, RAFAEL FELIPE; DORRESTEIN, PIETER C.; CAVALHEIRO, ALBERTO JOSE; BOLZANI, VANDERLAN S.. Can Statistical Evaluation Tools for Chromatographic Method Development Assist in the Natural Products Workflow? A Case Study on Selected Species of the Plant Family Malpighiaceae. Journal of Natural Products, v. 83, n. 11, p. 3239-3249, NOV 25 2020. (19/08477-7, 17/19702-6, 18/24865-4, 13/07600-3, 16/16970-7, 14/50926-0)

ALAN C. PILON; DENISE M. SELEGATO; RICHARD P. FERNANDES; PAULA C. P. BUENO; DANIELLE R. PINHO; FAUSTO CARNEVALE NETO; RAFAEL T. FREIRE; IAN CASTRO-GAMBOA; VANDERLAN S. BOLZANI; NORBERTO P. LOPES. METABOLÔMICA DE PLANTAS: MÉTODOS E DESAFIOS. Química Nova, v. 43, n. 3, p. 329-354, 2020-06-01. (13/07600-3, 14/05935-0, 19/15889-0, 17/17850-8, 14/50265-3, 17/19702-6, 15/50488-5, 19/08477-7, 14/50926-0, 16/13292-8)

MANNOCHIO-RUSSO, HELENA; DE ALMEIDA, RAFAEL F.; NUNES, WILHAN D. G.; BUENO, PAULA C. P.; CARABALLO-RODRIGUEZ, ANDRES M.; BAUERMEISTER, ANELIZE; DORRESTEIN, PIETER C.; BOLZANI, VANDERLAN S.. Untargeted Metabolomics Sheds Light on the Diversity of Major Classes of Secondary Metabolites in the Malpighiaceae Botanical Family. FRONTIERS IN PLANT SCIENCE, v. 13, p. 19-pg., 2022-04-14. (13/07600-3, 18/24865-4, 14/50926-0, 17/19702-6, 19/08477-7)