Advanced search
Start date
Betweenand

MATRIX-GNPS accelerated microbial biodiscovery

Grant number: 19/09350-0
Support type:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): August 20, 2019
Effective date (End): August 19, 2020
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Organic Chemistry
Principal researcher:Marcelo José Pena Ferreira
Grantee:Marcelo Marucci Pereira Tangerina
Supervisor abroad: Robert John Capon
Home Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Research place: University of Queensland, Brisbane (UQ), Australia  
Associated to the scholarship:17/16606-6 - Actinomycetes from Brazilian islands: a metabolomic approach in bioprospecting natural products with antitumor potential, BP.PD

Abstract

Environmental conditions drive adaptations in microbial secondary metabolism, which encodes a plethora of biosynthetic gene clusters (BGCs). Genomic discoveries reveals most of BCGs are silent (inactive) and keep an incredible chemical diversity yet to be explored. With the rising of antibiotic resistance, the need for new drug leads is as urgent as ever. This project aims to utilize modern cultivation conditions to transcriptionally activate silent BCGs in order to elicit production of novel metabolites by rare actinomycetes strains. For this purpose it will be utilized a combination of a miniaturised high-throughput microbial cultivation approach (MATRIX) and molecular networking (GNPS), along with antimicrobial evaluation against antibiotic resistant strains and cytotoxicity assays. After selection of the most promising strains and cultivation parameters, large scale fermentations and extracts fractionation and isolation of compounds will be performed. This approach utilizes a fast and cost-effective methodology towards novel and bioactive compounds.

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)