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Characterization of astroglial clonal responses in experimental animal models of brain injury

Grant number: 19/09183-7
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): July 22, 2019
Effective date (End): July 21, 2020
Field of knowledge:Biological Sciences - Morphology - Cytology and Cell Biology
Principal Investigator:Marimélia Aparecida Porcionatto
Grantee:Lina Maria Delgado Garcia
Supervisor abroad: Laura Lopez Mascaraque
Home Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Local de pesquisa : Instituto Cajal, Spain  
Associated to the scholarship:16/19084-8 - Crosstalk among Wnt, SHH and Notch and the efect of Gal3 protein on the regulation of the proliferation of derived-neural stem cells reactive astrocytes, BP.DR


Astrocytes are the first line of cellular response after a brain injury. To fulfil this role, astrocytes undergo a wide variety of alterations in gene expression and phenotype. Increasing evidence indicates that neurogenesis may have a regenerative role in response to brain injury and disease. Moreover, it has been suggested that, activation of signaling mechanisms regulating adult neural stem cells alone is enough to drive neurogenesis and neuroprotection. Given these scenarios, the purpose of this research project is to study astroglial clonal response and progeny identity using the StarTrack system in experimental animal models of brain injury. First, the StarTrack plasmid mixture will be transfected by in utero electroporation to label cells for tracking after induction of brain injury in adult mice. The injury models will be Experimental Autoimmune Encephalomyelitis (EAE) and the cuprizone model for demyelinitation, followed by astroglial clonal response analysis. Finally, we will evaluate SOX2 immunolabeling, spatial distribution and relation with the astrocyte-derived clones.