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Analysis of the whole transcriptome of co-cultured osteoblasts and osteoclasts grown on titanium surface with nanotopography

Grant number: 19/09349-2
Support Opportunities:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): October 01, 2019
Effective date (End): March 20, 2020
Field of knowledge:Health Sciences - Dentistry - Oral and Maxillofacial Surgery
Principal Investigator:Márcio Mateus Beloti
Grantee:Rayana Longo Bighetti Trevisan
Supervisor: Janet L. Stein
Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Research place: University of Vermont (UVM), United States  
Associated to the scholarship:17/23888-8 - Effect of titanium surface with nanotopography on the interaction between osteoblasts and osteoclasts, BP.DR


The osseointegration of titanium (Ti) implants is based on bone remodeling, which involves a balance between bone tissue formation by osteoblasts and tissue resorption by osteoclasts. Our group has shown that a Ti surface with nanotopography obtained by chemical conditioning with H2SO4/H2O2 solution is capable of inducing osteoblast differentiation under both osteogenic and non-osteogenic conditions. However, the effect of this surface on osteoclasts, and their interaction with osteoblasts have not been investigated yet. To understand the transcriptional changes caused by Ti surface with nanotopography during the interaction between osteoblastic and osteoclastic cells, we will evaluate by high-throughput RNA sequencing: (1) the effect of Ti with nanotopography surface (Ti-Nano), compared with control Ti surface, on osteoblastic and osteoclastic differentiation in isolated cultures of MC3T3-E1 and RAW 264.7 cells, respectively, grown on these surfaces, (2) the effect of MC3T3-E1 cells on osteoclastic differentiation of RAW 264.7 cells grown on Ti surface with nanotopography, compared with control Ti surface, in indirect co-culture models and (3) the effect of RAW 264.7 cells on osteoblastic differentiation of MC3T3-E1 cells grown on Ti surface with nanotopography, compared with control Ti surface, in indirect co-culture models. Differential expression analysis will be performed with DESeq2 considering fold change >2 to differential gene expression, and p-value <0.05. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TREVISAN, R. L. B.; FERRAZ, E. P.; GORDON, J. A. R.; TYE, C.; LIAN, J. B.; STEIN, G. S.; STEIN, J. L.; ROSA, A. L.; BELOTI, M. M.. Effects of Osteoclasts on Transcriptional Regulation of Non-Coding RNAs in Osteoblasts Grown on Titanium with Nanotopography. MOLECULAR BIOLOGY OF THE CELL, v. 34, n. 2, p. 2-pg., . (17/12622-7, 18/17356-6, 19/09349-2, 17/23888-8)
BIGHETTI-TREVISAN, RAYANA L.; ALMEIDA, LUCIANA O.; CASTRO-RAUCCI, LARISSA M. S.; GORDON, JONATHAN A. R.; TYE, CORALEE E.; STEIN, GARY S.; LIAN, JANE B.; STEIN, JANET L.; ROSA, ADALBERTO L.; BELOTI, MARCIO M.. Titanium with nanotopography attenuates the osteoclast-induced disruption of osteoblast differentiation by regulating histone methylation. BIOMATERIALS ADVANCES, v. 134, p. 13-pg., . (18/17356-6, 19/09349-2, 17/23888-8)

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